2-Fluoropalmitic acid (2-Fluorohexadecanoic acid), a fatty acid containing fluorine atoms, serves as a potential therapeutic agent for glioblastoma (GBM) by inhibiting the viability, proliferation, and stem-like phenotype of glioma stem cells (GSCs), suppressing the expression of phosphorylated erk, CD133, and SOX-2, and leading to decreased MMP-2 activity and increased methylation of the MGMT promoter.

2-Fluoropalmitic acid与人类白血病细胞系（如MOLT-4）和正常成纤维细胞（HDF细胞）孵育后，采用MTT法评估细胞存活率，并通过流式细胞术或荧光显微镜观察细胞凋亡情况。结果表明，2-Fluoropalmitic acid（浓度范围12.5-50μg/ml）对人类白血病细胞系（如MOLT-4）表现出显著的细胞毒性，但对正常成纤维细胞（HDF细胞）无明显毒性，对正常细胞的影响较小，同时能够诱导癌细胞凋亡[1]。