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2-Iminobiotin hydrobromide

产品编号 T7206L CAS 76985-52-9

别名: 2-亚氨基生物素, Guanidinobiotin, 2-亚氨基生物素盐酸盐

2-Iminobiotin hydrobromide (Guanidinobiotin) 是生物素 (维生素 H 或 B7) 类似物。它是一种可逆的 NO 合酶抑制剂，可作用于小鼠 iNOS(Ki:21.8 μM)和大鼠 n-cNOS(Ki:37.5 μM)。它和低温环境均可以保护人类神经细胞，使其免受缺氧诱导的细胞损伤。

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2-Iminobiotin hydrobromide Chemical Structure

2-Iminobiotin hydrobromide, CAS 76985-52-9

规格	价格/CNY	货期
1 mg	￥ 327	现货
2 mg	￥ 469	现货
5 mg	￥ 776	现货
10 mg	￥ 1,230	现货
25 mg	￥ 2,220	现货
50 mg	￥ 3,660	现货
100 mg	￥ 5,280	现货
1 mL * 10 mM (in DMSO)	￥ 961	现货

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其他形式的 2-Iminobiotin hydrobromide:

2-Iminobiotin
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生物活性

化学信息

存储 & 溶解度

参考文献

相关产品

产品描述	2-Iminobiotin hydrobromide (Guanidinobiotin) is a cyclic guanidino analog of biotin that acts as a reversible inhibitor of inducible nitric oxide synthase (iNOS) and neuronal NOS (nNOS; Ki of 21.8 and 37.5 μM for mouse iNOS and rat nNOS, respectively).
靶点活性	nNOS:37.5 µM(Ki)(rat), iNOS:21.8 µM(Ki) (mouse)
体外活性	All cultures were subjected to 25 h of hypothermia (33.5°C), and incubated with vehicle or 2-iminobiotin (2-IB) (10, 30, 50, 100, and 300 ng/ml).?Cell morphology was evaluated by brightfield microscopy.?Cell damage was analyzed by LDH assays.?Production of reactive oxygen species (ROS) was measured using fluorometric assays.?Western blotting for PARP, Caspase-3, and the phosphorylated forms of akt and erk1/2 was conducted.?To evaluate early apoptotic events and signaling, cell protein was isolated 4 h post-hypoxia and human apoptosis proteome profiler arrays were performed.?Twenty-five hour after the hypoxic insult, clear morphological signs of cell damage were visible and significant LDH release as well as ROS production were observed even under hypothermic conditions.?Post-hypoxic application of 2-IB (10 and 30 ng/ml) reduced the hypoxia-induced LDH release but not ROS production.?Phosphorylation of erk1/2 was significantly increased after hypoxia, while phosphorylation of akt, protein expression of Caspase-3 and cleavage of PARP were only slightly increased.?Addition of 2-IB did not affect any of the investigated proteins.?Apoptosis proteome profiler arrays performed with cellular protein obtained 4 h after hypoxia revealed that post-hypoxic application of 2-IB resulted in a ≥ 25% down regulation of 10/35 apoptosis-related proteins: Bad, Bax, Bcl-2, cleaved Caspase-3, TRAILR1, TRAILR2, PON2, p21, p27, and phospho Rad17[1].
细胞实验	In vitro hypoxia was induced for 7 h in IMR-32 cell cultures by using our recently described system with minor modifications.?Enzyme stock solutions (100x) of catalase and glucose oxidase ?were diluted in cell culture medium (DMEM/F12, 1% FCS;?final concentration: 120 and 2 U/ml respectively).?A rapid decrease of partial pressure of oxygen (pO2) to levels below 10 mmHg was achieved by adding the enzymes to glucose containing culture medium.?Also a decline in glucose (<1 g/l) and pH (<7.0) was observed, resembling the clinical characteristics of hypoxic-ischemic injury in vivo.?Hypoxic conditions were confirmed with a tissue oxygen pressure monitor .?After the hypoxic insult, cells were washed twice with PBS and cultures were placed into an incubator at 33.5°C (hypothermia) employing culture medium with (i) solvent (citrate buffer 1%) or (ii) 2-IB at 10, 30, 50, 100, and 300 ng/ml.?To determine the optimal “reperfusion” time, a time-interval curve investigating cell damage (LDH release) was performed.?Analyses of LDH release, ROS generation, hydrogen peroxide release, metabolic activity, cell signaling, apoptosis-related protein expression/activity and expression analysis of 35 human apoptosis-related proteins were performed at different time points post-hypoxia[1]

别名	2-亚氨基生物素, Guanidinobiotin, 2-亚氨基生物素盐酸盐
分子量	324.24
分子式	C10H18BrN3O2S
CAS No.	76985-52-9

存储

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

溶解度

DMSO: 12.5 mg/mL (51.37 mM)

溶液配制表

可选溶剂	浓度体积质量	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	3.0841 mL	15.4207 mL	30.8414 mL	77.1034 mL
	5 mM	0.6168 mL	3.0841 mL	6.1683 mL	15.4207 mL
	10 mM	0.3084 mL	1.5421 mL	3.0841 mL	7.7103 mL
	20 mM	0.1542 mL	0.771 mL	1.5421 mL	3.8552 mL
	50 mM	0.0617 mL	0.3084 mL	0.6168 mL	1.5421 mL

计算器

摩尔浓度计算器

稀释计算器

配液计算器

分子量计算器

质量

浓度

容积

分子量

浓度 (起始)

容积 (起始)

浓度 (终)

容积 (终)

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参考文献

1. Zitta K , Peeters-Scholte C , Sommer L , et al. 2-Iminobiotin Superimposed on Hypothermia Protects Human Neuronal Cells from Hypoxia-Induced Cell Damage: An in Vitro Study[J]. Frontiers in Pharmacology, 2018, 8:971. 2. Sup S J , Green B G , Grant S K . 2-Iminobiotin Is an Inhibitor of Nitric Oxide Synthases[J]. Biochemical & Biophysical Research Communications, 1994, 204(2):0-968.

剂量换算

对于不同动物的给药剂量换算，您也可以参考 更多...

体内实验配液计算器

请在以下方框中输入您的动物实验信息后点击计算，可以得到母液配置方法和体内配方的制备方法：比如您的给药剂量是10 mg/kg，每只动物体重20 g，给药体积100 μL，一共给药动物10 只，您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。

母液配置方法：2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL)，如您需要配置的浓度超过该产品的溶解度，请先与我们联系。

体内配方的制备方法：取 50 μL DMSO 主液，加入 300 μL PEG300，混匀澄清，再加 50 μL Tween 80，混匀澄清，再加 600 μL ddH2O, 混匀澄清。

第一步：请输入动物实验的基本信息

剂量

mg/kg

每只动物体重

给药体积

μL

动物数量

第二步：请输入动物体内配方组成，不同的产品配方组成不同，如有配方需求，可先联系我们提供正确的体内配方。

% DMSO+

% +

% Tween 80+

% ddH₂O

%DMSO+

计算重置

计算结果如下:

工作液浓度: mg/ml;

母液配置方法: mg 药物溶于 μL DMSO (母液浓度为 mg/mL)，如您需要配置的浓度超过该产品的溶解度，请先与我们联系。

体内配方的制备方法：取 μL DMSO 主液，加入 μL PEG300，混匀澄清，再加 μL Tween 80，混匀澄清，再加 μL ddH₂O, 混匀澄清。

备注:

要按顺序从左向右依次添加助溶剂。可配合物理方法，如涡流、超声波或热水浴使之帮助溶解。

技术支持

您可能有的问题的答案可以在抑制剂处理说明中找到，包括如何准备库存溶液，如何存储产品，以及基于细胞的分析和动物实验需要特别注意的问题。

Keywords

2-Iminobiotin hydrobromide 76985-52-9 Immunology/Inflammation NOS NO Synthase Nitric oxide synthases 2-Iminobiotin Hydrobromide 2-亚氨基生物素 Guanidinobiotin 2-Iminobiotin 2Iminobiotin hydrobromide 2 Iminobiotin hydrobromide Inhibitor inhibit 2-亚氨基生物素盐酸盐 inhibitor

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2-Iminobiotin hydrobromide

存储

溶解度

溶液配制表

计算器

输入分子式，点击计算，可计算出产品的分子量。

参考文献

相关产品

相关化合物库

剂量换算

体内实验配液计算器

技术支持

Keywords