Latent TGF beta 1 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 42.4 kDa and the accession number is A0A499FJK2.
规格 | 价格/CNY | 货期 | 数量 | |
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50 μg | ¥ 1,980 | 现货 | ||
100 μg | ¥ 3,450 | 5日内发货 |
生物活性 |
Measured by its binding ability in a functional ELISA. Immobilized Human TGF beta 1 His at 2 μg/ml (100 μl/well) can bind Human TGFBR2 His & hFc , the EC50 of Human TGFBR2 His & hFc is 5-35 ng/mL.
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产品描述 | Latent TGF beta 1 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 42.4 kDa and the accession number is A0A499FJK2. |
种属 | Human |
表达系统 | HEK293 Cells |
标签 | C-His |
蛋白编号 | A0A499FJK2 |
别名 | CED, DPD1, transforming growth factor, beta 1, TGF β1, LAP, 转化生长因子, TGFB, TGFbeta, TGF-beta 1, TGF-β1, TGFβ, transforming growth factor, β1 |
蛋白构建 | The full length of Human TGFβ1 (NP_000651.3) (Met 1-Ser 390) was fused with a C-terminal polyhistidine tag. |
蛋白纯度 |
> 95 % as determined by SDS-PAGE
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分子量 | 42.4 kDa (predicted) |
内毒素 | < 1.0 EU/μg of the protein as determined by the LAL method. |
缓冲液 | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
复溶方法 | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
存储 |
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
运输方式 |
In general, Lyophilized powders are shipping with blue ice. |
研究背景 | TGF-beta 1 is a member of the transforming growth factor beta (TGF-beta) family. The transforming growth factor-beta family of polypeptides are involved in the regulation of cellular processes, including cell division, differentiation, motility, adhesion and death. TGF-beta 1 positively and negatively regulates many other growth factors. It inhibits the secretion and activity of many other cytokines including interferon-γ, tumor necrosis factor-alpha and various interleukins. It can also decrease the expression levels of cytokine receptors. Meanwhile, TGF-beta 1 also increases the expression of certain cytokines in T cells and promotes their proliferation, particularly if the cells are immature. TGF-beta 1 also inhibits proliferation and stimulates apoptosis of B cells, and plays a role in controlling the expression of antibody, transferrin and MHC class II proteins on immature and mature B cells. As for myeloid cells, TGF-beta 1can inhibit their proliferation and prevent their production of reactive oxygen and nitrogen intermediates. However, as with other cell types, TGF-beta 1 also has the opposite effect on cells of myeloid origin. TGF-beta 1 is a multifunctional protein that controls proliferation, differentiation and other functions in many cell types. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts. Once cells lose their sensitivity to TGF-beta1-mediated growth inhibition, autocrine TGF-beta signaling can promote tumorigenesis. Elevated levels of TGF-beta1 are often observed in advanced carcinomas, and have been correlated with increased tumor invasiveness and disease progression.Cancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy |
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Latent TGF beta 1 Protein, Human, Recombinant (His) CED transforming growth factor, β 1 DPD 1 TGF-b 1 DPD1 transforming growth factor, beta 1 DPD-1 TGF β1 transforming growth factor, b 1 LAP TGF-β 1 转化生长因子 TGFB TGFbeta TGF-beta 1 TGF-β1 TGFβ transforming growth factor, β1 recombinant recombinant-proteins proteins protein