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CD73 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 59.2 kDa and the accession number is P21589-1.
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
50 μg | ¥ 4,120 | 现货 | |
100 μg | ¥ 6,190 | 5日内发货 | |
200 μg | ¥ 9,290 | 5日内发货 | |
500 μg | ¥ 15,860 | 5日内发货 |
生物活性 | 1. Using the Octet RED System, the affinity constant (Kd) of CD73 Protein, Human, Recombinant (His Tag) bound MEDI9447 was 0.3 nM._x000D_
2. Measured by its ability to hydrolyze the 5’phosphate group from the substrate adenosine 5’monophosphate (AMP). The specific activity is > 15,000 pmoles/min/μg. |
产品描述 | CD73 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 59.2 kDa and the accession number is P21589-1. |
种属 | Human |
表达系统 | HEK293 Cells |
标签 | C-His |
蛋白编号 | P21589-1 |
别名 | eN,NT5,E5NT,NT,5'-nucleotidase, ecto (CD73),NTE,CALJA,eNT,CD73 |
蛋白构建 | A DNA sequence encoding the human NT5E (NP_002517.1) (Met1-Lys547) was expressed fused with a polyhistidine tag at the C-terminus. Predicted N terminal: Trp 27 |
蛋白纯度 | > 85 % as determined by SDS-PAGE. > 95 % as determined by SEC-HPLC. |
分子量 | 59.2 kDa (predicted) |
内毒素 | < 1.0 EU/μg of the protein as determined by the LAL method. |
缓冲液 | Lyophilized from a solution filtered through a 0.22 μm filter, containing Tris, NaCl and CaCl2, pH 7.5, with Glycerol as protectants. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
复溶方法 | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
存储 | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
运输方式 | In general, Lyophilized powders are shipping with blue ice. |
研究背景 | 5'-nucleotidase, also known as NT5E, NTE, and CD73, is a cell membrane protein that belongs to the 5'-nucleotidase family. CD73 is a glycosylphosphatidylinositol (GPI) anchored purine salvage enzyme expressed on the surface of human T and B lymphocytes. CD73 catalyzes the conversion of purine and pyrimidine ribo- and deoxyribonucleoside monophosphates to the corresponding nucleosides. CD73 serves as a costimulatory molecule in activating T cells. CD73 generated adenosine functions in cell signaling in many physiologic systems, including intestinal epithelium, ischemic myocardium, and cholinergic synapses. CD73 might mediate lymphocyte-stromal cell interactions or condition the local microenvironment to facilitate lymphocyte development and/or function. In CD73-depleted cells, surface levels of the leukocyte adhesion molecules ICAM-1, VCAM-1, and E-selectin increase. CD73 produces extracellular adenosine, which then acts on G protein-coupled purinergic receptors to induce cellular responses. CD73 has also been reported to regulate the expression of pro-inflammatory molecules in mouse endothelium. |
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