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CD73 Protein, Human, Recombinant (His)

产品编号 TMPY-01851

CD73 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 59.2 kDa and the accession number is P21589-1.

CD73 Protein, Human, Recombinant (His)

CD73 Protein, Human, Recombinant (His)

产品编号 TMPY-01851
CD73 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 59.2 kDa and the accession number is P21589-1.
规格价格库存数量
50 μg¥ 4,120现货
100 μg¥ 6,1905日内发货
200 μg¥ 9,2905日内发货
500 μg¥ 15,8605日内发货
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生物活性

生物活性
1. Using the Octet RED System, the affinity constant (Kd) of CD73 Protein, Human, Recombinant (His Tag) bound MEDI9447 was 0.3 nM._x000D_ 2. Measured by its ability to hydrolyze the 5’phosphate group from the substrate adenosine 5’monophosphate (AMP). The specific activity is > 15,000 pmoles/min/μg.
CD73 Protein, Human, Recombinant (His)
产品描述
CD73 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 59.2 kDa and the accession number is P21589-1.
种属
Human
表达系统
HEK293 Cells
标签C-His
蛋白编号P21589-1
别名
eN,NT5,E5NT,NT,5'-nucleotidase, ecto (CD73),NTE,CALJA,eNT,CD73
蛋白构建
A DNA sequence encoding the human NT5E (NP_002517.1) (Met1-Lys547) was expressed fused with a polyhistidine tag at the C-terminus. Predicted N terminal: Trp 27
蛋白纯度
> 85 % as determined by SDS-PAGE. > 95 % as determined by SEC-HPLC.
CD73 Protein, Human, Recombinant (His)CD73 Protein, Human, Recombinant (His)
分子量59.2 kDa (predicted)
内毒素< 1.0 EU/μg of the protein as determined by the LAL method.
缓冲液Lyophilized from a solution filtered through a 0.22 μm filter, containing Tris, NaCl and CaCl2, pH 7.5, with Glycerol as protectants. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
复溶方法
A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
存储
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式In general, Lyophilized powders are shipping with blue ice.
研究背景
5'-nucleotidase, also known as NT5E, NTE, and CD73, is a cell membrane protein that belongs to the 5'-nucleotidase family. CD73 is a glycosylphosphatidylinositol (GPI) anchored purine salvage enzyme expressed on the surface of human T and B lymphocytes. CD73 catalyzes the conversion of purine and pyrimidine ribo- and deoxyribonucleoside monophosphates to the corresponding nucleosides. CD73 serves as a costimulatory molecule in activating T cells. CD73 generated adenosine functions in cell signaling in many physiologic systems, including intestinal epithelium, ischemic myocardium, and cholinergic synapses. CD73 might mediate lymphocyte-stromal cell interactions or condition the local microenvironment to facilitate lymphocyte development and/or function. In CD73-depleted cells, surface levels of the leukocyte adhesion molecules ICAM-1, VCAM-1, and E-selectin increase. CD73 produces extracellular adenosine, which then acts on G protein-coupled purinergic receptors to induce cellular responses. CD73 has also been reported to regulate the expression of pro-inflammatory molecules in mouse endothelium.

计算器

  • 复溶 计算器
  • 重组蛋白稀释 计算器
  • 比活力 计算器

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