CGP 25454A increases the field-stimulated [3H]- and [14C]-overflow from rat striatal slices preloaded with [3H]dopamine and [14C]choline, indicating that CGP 25454A is able to enhance the release of both dopamine (DA) and acetylcholine (ACh). However, CGP 25454A is 12.9 times more potent in increasing, by 1/6 of the apparent maximal increase, the release of [3H]DA than that of [14C]ACh.

CGP 25454A可使大鼠纹状体中D₂受体家族对[^3H]spiperone的结合增加90–110%（ED₅₀：13 mg/kg，腹腔注射）。
在30–100 mg/kg剂量范围内，CGP 25454A通过阻断突触后多巴胺（DA）受体，抑制同一动物垂体中[^3H]spiperone的结合。