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Retinoic acid

Retinoic acid

产品编号 T1051   CAS 302-79-4
别名: 维生素A酸, Vitamin A acid, ATRA, all-trans-Retinoic acid, Tretinoin

Retinoic acid (Tretinoin) 是维生素 A 的代谢产物,是一种视黄酸受体 RAR 的天然激动剂,抑制 RARα/β/γ (IC50=14 nM)。Retinoic acid 可以诱导细胞分化、减少细胞增殖和抑制肿瘤发生。

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Retinoic acid Chemical Structure
Retinoic acid, CAS 302-79-4
规格 价格/CNY 货期 数量
50 mg ¥ 298 现货
100 mg ¥ 418 现货
500 mg ¥ 629 现货
1 g ¥ 852 现货
1 mL * 10 mM (in DMSO) ¥ 460 现货
其他形式的 Retinoic acid:
产品目录号及名称: Retinoic acid (T1051)
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纯度: 99.52%
纯度: 97.21%
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天然产物信息
生物活性
化学信息
存储 & 溶解度
参考文献
结构类型
产品描述 Retinoic acid (Tretinoin), a metabolite of vitamin A, is a natural agonist of the retinoic acid receptor RAR and inhibits RARα/β/γ (IC50=14 nM). Retinoic acid induces cellular differentiation, reduces cellular proliferation, and inhibits tumorigenesis.
靶点活性 RARβ:14 nM, RARγ:14 nM, RARα:14 nM
体外活性 方法:人单核细胞 THP-1 用 Retinoic acid (10-100 nM) 处理 24 h,使用 Western Blot 方法检测靶点蛋白表达水平。
结果:Retinoic acid 处理的细胞中 RXRα 和 RARα 蛋白持续减少。[1]
方法:小鼠胚胎干细胞 mESC 用 Retinoic acid (1 μM) 处理 24 h,进行基因表达微阵列分析。
结果:Retinoic acid 处理后分化相关基因 Hoxb1、Hoxb2 和 Hoxb3 上调,而多能性相关基因 Oct4、Nanog、Klf4、Esrrb、Lefty1 和 Letfy2 下调。Retinoic acid 诱导ES细胞外胚层标志物表达。[2]
体内活性 方法:为研究对小鼠齿筋膜兴奋性和抑制性神经传递的影响,将 Retinoic acid (10 mg/kg in Corn oil+5% DMSO) 单次腹腔注射给 C57BL/6J 小鼠。
结果:在给予 Retinoic acid 6 h 后,背侧海马中的自发兴奋性突触后电流频率和突触数量均显著增加。Retinoic acid 介导小鼠齿状颗粒细胞中突触足蛋白依赖性化生。[3]
方法:为检测对小鼠肝脏脂质代谢的影响,将 Retinoic acid (10-100 mg/kg) 皮下注射给 NMRI 小鼠,每天一次,持续四天。
结果:Retinoic acid 治疗可减轻体重和肥胖。Retinoic acid 在体内将肝脏脂质代谢转变为增加分解代谢和减少脂肪生成。[4]
细胞实验 Retinoic acid is dissolved in DMSO and stored, and then diluted with appropriate medium before use[3]. P19 cell are induced to undergo neuronal differentiation according to established procedures. Briefly, cells are cultured on 1% agarose-coated 10 cm dishes at 3×10 5 cells/mL in α-minimal essential medium supplemented with 10% FBS. Differentiation is induced by addition of Retinoic acid (1 μM) and medium containing Retinoic acid replaced 2 days later. On day 4, cell aggregates are collected by centrifugation, separated to single cells by trypsin/EDTA treatment, replated onto poly-L-lysine-coated plates, and cultured in α-minimal essential medium supplemented with 10% FBS. On day 6, medium is replaced with neurobasal medium containing B27 supplement and 2 mM GlutaMAX. Medium is replaced every 2 days for an additional week[3].
别名 维生素A酸, Vitamin A acid, ATRA, all-trans-Retinoic acid, Tretinoin
分子量 300.44
分子式 C20H28O2
CAS No. 302-79-4

存储

keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year

溶解度

DMSO: 56 mg/mL (186.4 mM)

Ethanol: 6 mg/mL (19.97 mM)

H2O: < 1 mg/mL (insoluble or slightly soluble)

溶液配制表

可选溶剂 浓度 体积 质量 1 mg 5 mg 10 mg 25 mg
DMSO / Ethanol 1 mM 3.3285 mL 16.6423 mL 33.2845 mL 83.2113 mL
5 mM 0.6657 mL 3.3285 mL 6.6569 mL 16.6423 mL
10 mM 0.3328 mL 1.6642 mL 3.3285 mL 8.3211 mL
DMSO 20 mM 0.1664 mL 0.8321 mL 1.6642 mL 4.1606 mL
50 mM 0.0666 mL 0.3328 mL 0.6657 mL 1.6642 mL
100 mM 0.0333 mL 0.1664 mL 0.3328 mL 0.8321 mL

计算器

摩尔浓度计算器
稀释计算器
配液计算器
分子量计算器
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输入分子式,点击计算,可计算出产品的分子量。

参考文献

1. Chen Q, et al. Retinoic acid regulates cell cycle progression and cell differentiation in human monocytic THP-1 cells. Exp Cell Res. 2004 Jul 1;297(1):68-81. 2. Zhang J, et al. Retinoic Acid Induces Embryonic Stem Cell Differentiation by Altering Both Encoding RNA and microRNA Expression. PLoS One. 2015 Jul 10;10(7):e0132566. 3. Lenz M, et al. All-trans retinoic acid induces synaptopodin-dependent metaplasticity in mouse dentate granule cells. Elife. 2021 Nov 1;10:e71983. 4. Amengual J, et al. Retinoic acid treatment enhances lipid oxidation and inhibits lipid biosynthesis capacities in the liver of mice. Cell Physiol Biochem. 2010;25(6):657-66. 5. Muehlberger T, et al. J Am Acad Dermatol, 2005, 52(4), 583-588. 6. Wu L, et al. Retinoid X Receptor Agonists Upregulate Genes Responsible for the Biosynthesis of All-Trans-Retinoic Acid in Human Epidermis. PLoS One. 2016 Apr 14;11(4):e0153556. 7. Wan X Q, Cai J Y, Zhu Y, et al. SENP1 has an important role in lung development and influences the differentiation of alveolar type 2 cells[J]. International journal of molecular medicine. 2019 Jan;43(1):371-381. 8. Wei Z, Li T, Sun Y, et al. Daturataturin A, a withanolide in Datura metel L., induces HaCaT autophagy through the PI3K‐Akt‐mTOR signaling pathway[J] . Phytotherapy Research. 2021 9. Yanxing Wang, Xiaodong Dou, Lan Jiang, Hongwei Jin, Lihe Zhang, Liangren Zhang, and Zhenming Liu. Discovery of novel glycogen synthase kinase-3α inhibitors: Structurebased virtual screening, preliminary SAR and biological evaluation for treatment of acute myeloid leukemia [J]. European Journal of Medicinal Chemistry. 2019 Jun 1;171:221-234. 10. Qiu Y, Sun Y, Xu D, et al. Screening of FDA-approved drugs identifies sutent as a modulator of UCP1 expression in brown adipose tissue[J]. EBioMedicine. 2018, 37: 344-355.

文献引用

1. Qiu Y, Sun Y, Xu D, et al. Screening of FDA-approved drugs identifies sutent as a modulator of UCP1 expression in brown adipose tissue. EBioMedicine. 2018, 37: 344-355. 2. Wang Y, Dou X, Jiang L, et al. Discovery of novel glycogen synthase kinase-3α inhibitors: Structurebased virtual screening, preliminary SAR and biological evaluation for treatment of acute myeloid leukemia. European Journal of Medicinal Chemistry. 2019, 171: 221-234 3. Wei Z, Li T, Sun Y, et al. Daturataturin A, a withanolide in Datura metel L., induces HaCaT autophagy through the PI3K‐Akt‐mTOR signaling pathway. Phytotherapy Research. 2021 Mar;35(3):1546-1558. doi: 10.1002/ptr.6921. Epub 2021 Feb 9. 4. Wan X Q, Cai J Y, Zhu Y, et al. SENP1 has an important role in lung development and influences the differentiation of alveolar type 2 cells. International journal of molecular medicine. 2019 Jan;43(1):371-381. 5. Zhu Y, Gu X, Wang L, et al.All-Trans Retinoic Acid Promotes M2 Macrophage Polarization in Vitro by Activating the p38MAPK/STAT6 Signaling Pathway.Immunological Investigations.2023: 1-21.
UVI 3003 Etodolac BMS 195614 Ro 41-5253 Ch55 HX 531 Retinoic acid-d5 AGN 193109

相关化合物库

该产品包含在如下化合物库中:
抗癌药物库 抗癌活性化合物库 抗癌临床化合物库 抗癌上市药物库 上市药物库 抗代谢疾病化合物库 免疫/炎症分子化合物库 抗胰腺癌化合物库 表型筛选靶点鉴定库 NO PAINS 化合物库

剂量换算

对于不同动物的给药剂量换算,您也可以参考 更多...

体内实验配液计算器

请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。

母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。

体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。

第一步:请输入动物实验的基本信息
剂量
mg/kg
每只动物体重
g
给药体积
μL
动物数量
第二步:请输入动物体内配方组成,不同的产品配方组成不同,如有配方需求,可先联系我们提供正确的体内配方。
% DMSO
%
% Tween 80
% ddH2O
计算 重置

技术支持

您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。

Keywords

Retinoic acid 302-79-4 Autophagy DNA Damage/DNA Repair Metabolism Retinoid Receptor Endogenous Metabolite PPAR 维生素A酸 Vitamin A acid ATRA inhibit all-trans-Retinoic acid RAR/RXR Tretinoin Inhibitor Retinoic acid receptors Retinoid X receptors Peroxisome proliferator-activated receptors inhibitor

 

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