Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Retapamulin (SB-275833) 是一种能结合金黄色葡萄球菌和大肠杆菌核糖体的局部抗生素,Kd 为3 nM。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
1 mg | ¥ 181 | 现货 | ||
5 mg | ¥ 397 | 现货 | ||
10 mg | ¥ 659 | 现货 | ||
50 mg | ¥ 997 | 现货 | ||
100 mg | ¥ 1,580 | 现货 | ||
200 mg | ¥ 2,380 | 现货 | ||
500 mg | ¥ 3,950 | 现货 | ||
1 mL * 10 mM (in DMSO) | ¥ 725 | 现货 |
产品描述 | Retapamulin (SB-275833), a Pleuromutilin Antibacterial, binds to both E. coli and S. aureus ribosomes with similar potencies (Kd: 3 nM). |
靶点活性 | Ribosome:3 nM(Kd) |
体外活性 | Retapamulin is a potent inhibitor of protein synthesis with an IC50 of 0.33 μM in lysates prepared from erythromycin-susceptible E. coli cells. Retapamulin (100 μM) is ineffective in inhibiting eukaryotic translation when tested in a rabbit reticulocyte lysate system with the cellular components necessary for mammalian protein synthesis. Retapamulin binds to Erys ribosomes and fully displaces the labeled ligand with an IC50 of 26.1 nM. Retapamulin partially inhibits the ability of charged, N-blocked tRNA to bind to the P-site of E. coli ribosomes, with an IC50 of 17.4 nM (maximum inhibition of 80%). [1] Retapamulin inhibits Staphylococcus aureus and Streptococcus pyogenes with MIC90 of 0.12 μg/mL and ≤0.03 μg/mL, respectively. Retapamulin inhibits S. aureus subset with MIC50/90 values of 0.06/0.12 μg/mL. Retapamulin shows excellent activity against these isolates, with only two requiring a MIC of 0.06 μg/mL. [2] Retapamulin is very active against the S. pyogenes isolates tested with MIC90 of 0.016 μg/mL, and based on MIC90s, is 32- and >1,024-fold more active than mupirocin and fusidic acid, respectively. Retapamulin binds to a unique site on the bacterial ribosome, and by virtue of its novel mode of action. [3] Retapamulin (<2 mg/L) inhibits 37/52 (71%) strains of the B. fragilis group and 85/87 (98%) of the other Gram-negative bacilli. Retapamulin is more active than clindamycin, metronidazole and ceftriaxone against Propionibacterium acnes and anaerobic Gram-positive cocci. [4] Retapamulin inhibits total viable cells (TVC), Protein synthesis and 50S subunit synthesis in both wild-type (wt) Staphylococcus aureus strain RN1786 with IC50 of 12 ng/mL, 5 ng/mL and 27 ng/mL, respectively. [5] |
别名 | 瑞他莫林, SB-275833 |
分子量 | 517.76 |
分子式 | C30H47NO4S |
CAS No. | 224452-66-8 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 104 mg/mL (200.86 mM)
Ethanol: 104 mg/mL (200.86 mM)
H2O: < 1 mg/mL (insoluble or slightly soluble)
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
DMSO / Ethanol | 1 mM | 1.9314 mL | 9.657 mL | 19.314 mL | 48.2849 mL |
5 mM | 0.3863 mL | 1.9314 mL | 3.8628 mL | 9.657 mL | |
10 mM | 0.1931 mL | 0.9657 mL | 1.9314 mL | 4.8285 mL | |
20 mM | 0.0966 mL | 0.4828 mL | 0.9657 mL | 2.4142 mL | |
50 mM | 0.0386 mL | 0.1931 mL | 0.3863 mL | 0.9657 mL | |
100 mM | 0.0193 mL | 0.0966 mL | 0.1931 mL | 0.4828 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
Retapamulin 224452-66-8 Microbiology/Virology ribosome Antibiotic Antibacterial Bacterial 瑞他莫林 inhibit SB 275833 SB275833 Inhibitor SB-275833 inhibitor