Powder: -20°C for 3 years | In solvent: -80°C for 1 year
RG108 (N-Phthalyl-L-tryptophan) 是一种非核苷的 DNA 甲基转移酶 (DNMTs) 抑制剂,IC50为115 nM。它阻断 DNA 甲基转移酶的活性位点,可引起抑癌基因的去甲基化和再激活。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
1 mg | ¥ 196 | 现货 | ||
5 mg | ¥ 435 | 现货 | ||
10 mg | ¥ 688 | 现货 | ||
25 mg | ¥ 1,470 | 现货 | ||
50 mg | ¥ 2,930 | 现货 | ||
100 mg | ¥ 4,220 | 现货 | ||
200 mg | ¥ 5,910 | 现货 | ||
500 mg | ¥ 8,830 | 现货 | ||
1 mL * 10 mM (in DMSO) | ¥ 497 | 现货 |
产品描述 | RG108 (N-Phthalyl-L-tryptophan) is an DNA methyltransferase inhibitor(IC50=115 nM). |
靶点活性 | DNA methyltransferase:115 nM |
体内活性 | SM衍生的iPS细胞中,RG108能够明显降低DNA活性。在肿瘤细胞中,RG108抑制基因脱甲基和再活化,但是不影响着丝粒基因序列的甲基化。 |
激酶实验 | In vitro methylation assay: The substrate DNA for the in vitro methylation assay is a 798 bp fragment (?423/+375 relative to the initiation codon) from the promoter region of the human p16Ink4a gene. The methylation reaction contains 350 to 400 ng substrate DNA and 4 units of M.SssI methylase (0.5 μM) in a final volume of 50 μL. Inhibitors are added to final concentrations of 10, 100, 200, and 500 μM, respectively. Reactions are done at 37 °C for 2 hours. After completion, the reaction is inactivated at 65 °C for 15 minutes and the DNA is purified using PCR Purification kit. Three hundred nanograms of purified DNA is digested for 3 hours at 60 °C with 30 units of BstUI and analyzed on 2% Tris-borate EDTA agarose gels. |
细胞实验 | For the determination of cellular growth and viability, cells are stained with trypan blue and counted using a standard counting grid.(Only for Reference) |
别名 | N-Phthalyl-L-tryptophan |
分子量 | 334.33 |
分子式 | C19H14N2O4 |
CAS No. | 48208-26-0 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Ethanol: 33.4 mg/mL (100 mM)
DMSO: 33.4 mg/mL (100 mM)
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
Ethanol / DMSO | 1 mM | 2.9911 mL | 14.9553 mL | 29.9106 mL | 74.7764 mL |
5 mM | 0.5982 mL | 2.9911 mL | 5.9821 mL | 14.9553 mL | |
10 mM | 0.2991 mL | 1.4955 mL | 2.9911 mL | 7.4776 mL | |
20 mM | 0.1496 mL | 0.7478 mL | 1.4955 mL | 3.7388 mL | |
50 mM | 0.0598 mL | 0.2991 mL | 0.5982 mL | 1.4955 mL | |
100 mM | 0.0299 mL | 0.1496 mL | 0.2991 mL | 0.7478 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
RG108 48208-26-0 Chromatin/Epigenetic DNA Methyltransferase inhibit Inhibitor DNMTs N-Phthalyl-L-tryptophan DNA MTases RG 108 RG-108 inhibitor