Powder: -20°C for 3 years | In solvent: -80°C for 2 years
Nigericin sodium salt 是来自吸水链霉菌的抗生素,是一种 NLRP3 激活剂。Nigericin sodium 是一种阳离子离子载体,可抑制高尔基体功能并抑制革兰氏阳性菌的生长。 它还可以防止病毒激活并触发 NALP3 炎性体的激活。
产品描述 | Nigericin sodium salt is an antibiotic from Streptomyces hygroscopicus and is a NLRP3 activator.Nigericin sodium is a cationic ionophore that inhibits Golgi function and suppresses growth of gram positive bacteria. It also prevents viral activation and triggers activation of the NALP3 inflammasome. |
体内活性 | A 48-hr exposure of human erythrocytes to nigericin significantly increased the percentage of annexin-V-binding cells (0.1-10 nM), significantly decreased forward scatter (0.1-1 nM), significantly decreased cytosolic pH (0.1-1 nM) and significantly increased Fluo3 fluorescence (0.1-10 nM).?Nigericin (1 nM) slightly, but significantly, increased ROS, but did not significantly modify ceramide abundance.?The effect of nigericin on annexin V binding was significantly blunted, but not abolished by removal of extracellular Ca(2+).?The nigericin-induced increase in [Ca(2+)]i and annexin V binding was again significantly blunted but not abolished by the Na(+)/H(+) exchanger inhibitor cariporide (10 μM).?Nigericin triggers eryptosis, an effect paralleled by ROS formation, in part dependent on stimulation of Ca(2+) entry, and involving the cariporide-sensitive Na(+)/H(+) exchanger[1]. |
别名 | 尼日利亚菌素, 尼日利亚菌素钠盐 |
分子量 | 746.94 |
分子式 | C40H67NaO11 |
CAS No. | 28643-80-3 |
Powder: -20°C for 3 years | In solvent: -80°C for 2 years
DMSO: 3mg/mL(4.02 mM), Need ultrasonic
Methanol: 122 mg/mL (163.33mM)
Ethanol: 47 mg/mL (62.92mM)
( < 1 mg/mL refers to the product slightly soluble or insoluble )
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
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您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
Nigericin sodium salt 28643-80-3 Immunology/Inflammation Membrane transporter/Ion channel Microbiology/Virology NF-Κb NOD-like Receptor (NLR) NOD Antibiotic Potassium Channel Antibacterial Inhibitor Nigericin Bacterial 尼日利亚菌素 尼日利亚菌素钠盐 inhibit KcsA inhibitor