Name: EGFR Protein, Human, Recombinant (His)
Brand: TargetMol
SKU: TMPY-00742
Price: 493 CNY
Availability: InStock
Rating: 5 (10 reviews)

规格

价格

库存

数量

5 μg

¥ 493

现货

10 μg

¥ 788

现货

20 μg

¥ 1,330

3日内发货

50 μg

¥ 2,630

3日内发货

100 μg

¥ 4,450

现货

200 μg

¥ 7,970

5日内发货

500 μg

¥ 17,300

5日内发货

生物活性

产品描述	EGFR Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 69.8 kDa and the accession number is P00533-1.
生物活性	1.Measured by its binding ability in a functional ELISA. Immobilized Human EGF hFc at 2 μg/ml (100 μl/well) can bind Human EGFR His , the EC50 of Human EGFR His is 100-500 ng/mL. 2. Captured Cetuximab (IgG1) on proA Chip can bind Human EGFR recombinant protein with an affinity constant of 1.07 nM as determined in an SPR assay (Routinely tested). 3. Loaded Cetuximab on ProA Biosensor, can bind Human EGFR protein, His Tag with an affinity constant of 5.81nM as determined in BLI assay (Routinely tested).
研究背景	The epidermal growth factor receptor is a transmembrane protein that is a receptor for members of the epidermal growth factor family of extracellular protein ligands. The epidermal growth factor receptor is a member of the ErbB family of receptors, a subfamily of four closely related receptor tyrosine kinases: EGFR, HER2/neu, Her 3 and Her 4. Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses.
种属	Human
表达系统	HEK293 Cells
标签	C-His
蛋白编号	P00533-1
蛋白构建	A DNA sequence encoding the extracellular domain (Met 1-Ser 645) of human EGFR (NP_005219) was expressed with a C-terminal polyhistidine tag. Predicted N terminal: Leu 25
蛋白纯度	> 95 % as determined by SDS-PAGE. >90% as determined by SEC-HPLC
蛋白性状	Lyophilized powder
缓冲液	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
复溶方法	Reconstituted with sterile deionized water to 0.25 mg/mL. Reconstitution conditions may vary depending on the lot.
别名	PIG61, NISBD2, mENA, HER1, ERBB1, ERBB, epidermal growth factor receptor
内毒素	< 1.0 EU/μg of the protein as determined by the LAL method.