p53 Activator 2 (compound 10ah) intercalates into DNA, causing significant DNA double-strand breaks and upregulates the expression of p53, p-p53, CDK4, and p21, leading to cell cycle arrest at the G2/M phase. It induces apoptosis, significantly reduces the expression of anti-apoptosis proteins Bcl-2, Bcl-xL, and cyclin B1 levels, and exhibits anti-proliferative activity against MGC-803 cells with an IC50 of 1.73 μM. Furthermore, p53 Activator 2 demonstrates potent anticancer effectiveness in MGC-803 xenograft tumor models [1].