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Anti-Phospho-RAF1 (Ser621) Antibody (2K681)
还可以产品编号 TMAH-00955
别名 Phospho-RAF1 (S621)
Anti-Phospho-RAF1 (Ser621) Antibody (2K681) 是一种抗体，靶向 Phospho-RAF1 (Ser621)。Anti-Phospho-RAF1 (Ser621) Antibody (2K681) 可用于 ELISA, WB, IF。
Anti-Phospho-RAF1 (Ser621) Antibody (2K681)
Anti-Phospho-RAF1 (Ser621) Antibody (2K681)
还可以产品编号 TMAH-00955 别名 Phospho-RAF1 (S621)
Anti-Phospho-RAF1 (Ser621) Antibody (2K681) 是一种抗体，靶向 Phospho-RAF1 (Ser621)。Anti-Phospho-RAF1 (Ser621) Antibody (2K681) 可用于 ELISA, WB, IF。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,300 | 5日内发货 | |
| 100 μL | ¥ 2,190 | 5日内发货 |
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| 产品描述 | Anti-Phospho-RAF1 (Ser621) Antibody (2K681) is an antibody targeting Phospho-RAF1 (Ser621). Anti-Phospho-RAF1 (Ser621) Antibody (2K681) can be used in ELISA, WB, IF. |
| 别名 | Phospho-RAF1 (S621) |
| Ig Type | Rabbit IgG |
| 克隆号 | 2K681 |
| 交叉反应 | Human |
| 验证活性 | 1. Western Blot -Positive WB detected in Hela whole cell lysate(treated with Calyculin A or EGF) -All lanes Phospho-RAF1 antibody at 1.525μg/ml -Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution -Predicted band size: 73 KDa -Observed band size: 73 KDa 2. Immunofluorescence staining of HepG2 cells(treated with 50mM Calyculin A for 30min) with TMAH-00955 at 1:100,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L). |
| 应用 | ELISA, WB, IF |
| 推荐剂量 | WB:1:500-1:5000; IF:1:20-1:200. |
| 抗体种类 | Monoclonal |
| 亚细胞定位 | Cytoplasm. Cell membrane. Mitochondrion. Nucleus. Note=Colocalizes with RGS14 and BRAF in both the cytoplasm and membranes. Phosphorylation at Ser-259 impairs its membrane accumulation. Recruited to the cell membrane by the active Ras protein. Phosphorylation at Ser-338 and Ser-339 by PAK1 is required for its mitochondrial localization. Retinoic acid-induced Ser-621 phosphorylated form of RAF1 is predominantly localized at the nucleus. |
| 构建方式 | Recombinant Antibody |
| 纯化方式 | Affinity-chromatography |
| 性状 | Liquid |
| 缓冲液 | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| 研究背景 | Serine/threonine-protein kinase that acts as a regulatory link between the membrane-associated Ras GTPases and the MAPK/ERK cascade, and this critical regulatory link functions as a switch determining cell fate decisions including proliferation, differentiation, apoptosis, survival and oncogenic transformation. RAF1 activation initiates a mitogen-activated protein kinase (MAPK) cascade that comprises a sequential phosphorylation of the dual-specific MAPK kinases (MAP2K1/MEK1 and MAP2K2/MEK2) and the extracellular signal-regulated kinases (MAPK3/ERK1 and MAPK1/ERK2). The phosphorylated form of RAF1 (on residues Ser-338 and Ser-339, by PAK1) phosphorylates BAD/Bcl2-antagonist of cell death at 'Ser-75'. Phosphorylates adenylyl cyclases: ADCY2, ADCY5 and ADCY6, resulting in their activation. Phosphorylates PPP1R12A resulting in inhibition of the phosphatase activity. Phosphorylates TNNT2/cardiac muscle troponin T. Can promote NF-kB activation and inhibit signal transducers involved in motility (ROCK2), apoptosis (MAP3K5/ASK1 and STK3/MST2), proliferation and angiogenesis (RB1). Can protect cells from apoptosis also by translocating to the mitochondria where it binds BCL2 and displaces BAD/Bcl2-antagonist of cell death. Regulates Rho signaling and migration, and is required for normal wound healing. Plays a role in the oncogenic transformation of epithelial cells via repression of the TJ protein, occludin (OCLN) by inducing the up-regulation of a transcriptional repressor SNAI2/SLUG, which induces down-regulation of OCLN. Restricts caspase activation in response to selected stimuli, notably Fas stimulation, pathogen-mediated macrophage apoptosis, and erythroid differentiation. |
| 偶联 | Unconjugated |
| 免疫原 | A synthetic peptide: Human Phospho-RAF1 (S621) |
| 抗原种属 | Human |
| 基因ID | 5894 |
| Uniprot ID | |
| 研究领域 | Signal Transduction |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
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