Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Sulforaphane 是存在于多种蔬菜中的一种天然异硫氰酸酯。它可以激活Nrf2,并通过 AMPK 依赖性信号传导抑制高糖诱导的胰腺癌。它增加肿瘤抑制蛋白的转录并抑制组蛋白脱乙酰酶的活性,具有抗癌和抗炎活性。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
5 mg | ¥ 297 | 现货 | ||
10 mg | ¥ 492 | 现货 | ||
25 mg | ¥ 1,120 | 现货 | ||
50 mg | ¥ 1,920 | 现货 | ||
100 mg | ¥ 3,730 | 现货 | ||
500 mg | ¥ 7,880 | 现货 |
产品描述 | Sulforaphane is derived from the consumption of cruciferous vegetables, such as broccoli. It has shown anticancer and cardioprotective activities. |
体外活性 | Sulforaphane以剂量依赖的方式引起细胞周期阻滞,随后导致细胞死亡。这种由sulforaphane引起的细胞周期阻滞与cyclins A和B1表达增加有关。Sulforaphane通过凋亡过程诱导细胞死亡,并抑制HT29静息结肠癌细胞的生长重启及降低细胞活性,对分化的CaCo2细胞毒性较低[1]。H9c2大鼠心肌细胞预处理sulforaphane可减少凋亡细胞数和凋亡蛋白表达,并降低多柔比星引起的线粒体膜电位增加。此外,sulforaphane增加血红素加氧酶-1的mRNA和蛋白表达,从而降低多柔比星引起的线粒体反应性氧种类水平[2]。 |
体内活性 | 给予sulforaphane能够降低单剂量DMBA诱发的雌性SD大鼠乳腺肿瘤的发生率、数量、体重,并延缓其发展[3]。 |
细胞实验 | HT29 cells are seeded at low density (5×10^4 cells/mL) in 35- or 120-mm diameter Primaria dishes in standard medium containing 5% FCS. One day after seeding, the medium is changed, and HT29 cells are treated with sulforaphane (0-30 μM). An equivalent amount of the solvent (ethanol) is added to control cells (0.2% final concentration). The drug effect on cellular viability is evaluated using the MTT assay [1]. |
动物实验 | At age 47, 48, 49, 50, and 51 days, each animal receives by gavage either 0.5 mL of Emulphor EL-620 alone or the specified doses (75, 100, or 150 μM daily) of sulforaphane or compound 2, 3, or 4 in 0.5 mL of Emulphor EL-620. On day 50, 3 hr after administration of the vehicle or protector, all rats also receive an intragastric instillation of 8.0 mg of DMBA dissolved in 1.0 mL of sesame oil. This dose of DMBA is selected to produce a substantial tumor incidence, but not one so high as to overwhelm a potential chemoprotective effect. The animals are examined once weekly for the appearance and location of palpable tumors. At age 202 days, i.e., 152 days after carcinogen administration, all animals are euthanized with ether and weighed. The tumors are separated from the fat and connective tissue by dissection weighed and fixed in buffered 10% formalin. All tumors are identified microscopically by examination of stained sections [3]. |
别名 | 萝卜硫素 |
分子量 | 177.29 |
分子式 | C6H11NOS2 |
CAS No. | 4478-93-7 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 60 mg/mL (338.43 mM)
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
DMSO | 1 mM | 5.6405 mL | 28.2024 mL | 56.4048 mL | 141.0119 mL |
5 mM | 1.1281 mL | 5.6405 mL | 11.281 mL | 28.2024 mL | |
10 mM | 0.564 mL | 2.8202 mL | 5.6405 mL | 14.1012 mL | |
20 mM | 0.282 mL | 1.4101 mL | 2.8202 mL | 7.0506 mL | |
50 mM | 0.1128 mL | 0.564 mL | 1.1281 mL | 2.8202 mL | |
100 mM | 0.0564 mL | 0.282 mL | 0.564 mL | 1.4101 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
Sulforaphane 4478-93-7 Apoptosis Chromatin/Epigenetic DNA Damage/DNA Repair Immunology/Inflammation Nrf2 HDAC 萝卜硫素 Keap1-Nrf2 inhibit Inhibitor Histone deacetylases inhibitor