Cisplatin

规格	价格/CNY		数量
100 mg	￥ 348	现货
500 mg	￥ 918	现货
1 g	￥ 1,543	现货

规格

价格/CNY

货期

数量

100 mg

￥ 348

现货

500 mg

￥ 918

现货

1 g

￥ 1,543

现货

存储

keep away from direct sunlight　|　Powder: -20°C for 3 years | In solvent: -80°C for 1 year

溶解度

H2O: 3.33mg/ml(11.1mM), Sonication is recommended. (DMSO inactivates the activity of Cisplatin.)

DMF: 20mg/ml(66.7mM)

溶液配制表

可选溶剂	浓度体积质量	1 mg	5 mg	10 mg	25 mg
H2O / DMF	1 mM	3.3329 mL	16.6644 mL	33.3289 mL	83.3222 mL
	5 mM	0.6666 mL	3.3329 mL	6.6658 mL	16.6644 mL
	10 mM	0.3333 mL	1.6664 mL	3.3329 mL	8.3322 mL
DMF	20 mM	0.1666 mL	0.8332 mL	1.6664 mL	4.1661 mL
DMF	50 mM	0.0667 mL	0.3333 mL	0.6666 mL	1.6664 mL

产品描述	Cisplatin (CDDP) is a DNA cross-linking agent. Cisplatin has antitumor activity and inhibits DNA synthesis by forming DNA adducts in cancer cells. Cisplatin also activates iron death and induces autophagy.
体外活性	方法：人非小细胞肺癌细胞 A549、SKMES-1、MOR 和 H460 用 Cisplatin (0.001-100 μM) 处理 72 h，使用 MTT 方法检测细胞生长抑制情况。结果：Cisplatin 剂量依赖性地抑制 A549、SKMES-1、MOR 和 H460 细胞生长，IC50 分别为 1.58 µM、4.09 µM、6.39 µM 和 5.72 µM。[1] 方法：人乳腺癌 MCF-7 和 MDA-MB-231 用 Cisplatin (2-10 μg/mL) 处理 48 h，使用 Western Blot 方法检测靶点蛋白表达水平。结果：Cisplatin 剂量依赖性诱导 MCF-7 和 MDA-MB-231 细胞中凋亡相关蛋白 cleaved-caspase 3 和 cleaved-PARP 的水平增加。[2] 方法：间皮瘤细胞 JU77、LO68 和 ONE58 用 Cisplatin (5-100 μg/mL) 处理 24 h，使用 JC-1 染料检测线粒体膜电位 (MMP)。结果：Cisplatin 剂量依赖性降低 JU77、LO68 和 ONE58 细胞的 MMP，抑制线粒体功能。[3]
体内活性	方法：为检测体内抗肿瘤活性，将 Cisplatin (5 mg/kg/6 天) 和 Chloroquine (13 mg/kg/天) 腹腔注射给携带咽下鳞状细胞癌肿瘤 (HSCC) FaDu 的 BALB/c nude 小鼠，持续十八天。结果：Cisplatin 治疗显著抑制 HSCC 肿瘤生长，Chloroquine 抑制自噬并增加 Cisplatin 诱导的细胞凋亡，从而增强了 Cisplatin 的疗效，导致小鼠的肿瘤生长减少和生存期延长。[4] 方法：为减轻 Cisplatin 治疗引起的肾毒性，将 Cisplatin (3-6 mg/kg/3天) 腹腔注射和 Cilastatin (100 mg/kg/天) 皮下注射给人肺腺癌肿瘤 A549 的 BALB/c 小鼠，持续七天。结果：Cilastatin 可以在不影响 Cisplatin 抗肿瘤作用的情况下减少其诱导的肾毒性。[5]
细胞实验	Rabbit renal proximal tubules were isolated using the iron oxide perfusion method and grown in 35-mm tissue culture dishes under improved conditions as described previously. The cell culture medium was a 1:1 mixture of Dulbecco's modified Eagle's medium/Ham's F-12 (without D-glucose, phenol red, or sodium pyruvate) supplemented with 15 mM HEPES buffer, 2.5 mM L-glutamine, 1 μM pyridoxine HCl, 15 mM sodium bicarbonate, and 6 mM lactate. Hydrocortisone (50 nM), selenium (5 ng/ml), human transferrin (5 μg/ml), bovine insulin (10 nM), and L-ascorbic acid-2-phosphate (50 μM) were added to fresh culture medium immediately before daily media change. In general, confluent RPTCs were treated with inhibitors or diluent control [typically DMSO at 0.1% (v/v)] for 30 min before treatment with cisplatin. Aliquots of RPTCs were used for various assays as detailed below [1].
动物实验	Mice were divided randomly into three groups (Control, Cisplatin and Cisplatin+HemoHIM), and each group consisted of twenty mice. B16F0 melanoma (5 × 10^5 cells/mouse) was inoculated into subcutaneous femoral left region of mice at 3 days before an initial injection of cisplatin. Cisplatin was injected intraperitoneally at 4 mg/kg body weight (B.W.) on day 0, 7 and 14 (total three injections). Experimental group was intubated with HemoHIM at a final concentration of 100 mg/kgB.W. by everyday from day -1 to day 16, while the control group received only water. On day 17 after initial injection of cisplatin, all mice of each group were experimented, respectively, to evaluate tumor weight or tumor size. The tumor size was calculated as follows: tumor size = ab^2/2, where a and b are the larger and smaller diameters, respectively [3].

别名	cis-Diaminodichloroplatinum, CDDP, 顺铂
化合物与蛋白结合的复合物	Solution Structures of a DNA Dodecamer Duplex with a Cisplatin 1,2-d(GG) Intrastrand Cross-Link
分子量	300.04
分子式	Cl2H6N2Pt
CAS No.	15663-27-1

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