Name: Cisplatin
Brand: TargetMol
SKU: T1564
Price: 116 CNY
Availability: InStock
Rating: 5 (10 reviews)

Cisplatin

还可以

产品编号 T1564Cas号 15663-27-1

别名 顺铂, cis-Diaminodichloroplatinum, CDDP

Cisplatin（CDDP）是一种具有抗肿瘤活性的化疗药物，是典型的DNA交联剂，能够通过在癌细胞中形成DNA加合物，抑制DNA合成并引起DNA损伤，从而导致细胞死亡。除此之外，Cisplatin 还可激活铁死亡（ferroptosis）并诱导自噬（autophagy）。在动物实验中，Cisplatin 常被用于构建慢性肾损伤和急性肾衰竭模型。

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Cisplatin

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纯度: 99.63%

产品编号 T1564 别名 顺铂, cis-Diaminodichloroplatinum, CDDPCas号 15663-27-1

规格	价格	库存
10 mg	¥ 116	In stock
50 mg	¥ 232	In stock
100 mg	¥ 348	In stock
500 mg	¥ 918	In stock
1 g	¥ 1,543	In stock

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产品介绍

生物活性

产品描述	Cisplatin (CDDP) is a chemotherapeutic agent with antitumor activity and is a classic DNA crosslinker. It inhibits DNA synthesis and induces DNA damage in cancer cells by forming DNA adducts, ultimately leading to cell death. In addition, Cisplatin can activate ferroptosis and induce autophagy. In animal studies, it is commonly used to establish models of chronic kidney injury and acute renal failure.
靶点活性	A549 cells:1.58 µM, MCF7 cells:1.2 µM, HCC38 cells:2.5 µM, SKMES-1 cells:4.09 µM
体外活性	方法：人非小细胞肺癌细胞 A549、SKMES-1、MOR 和 H460 用 Cisplatin (0.001-100 μM) 处理 72 h，使用 MTT 方法检测细胞生长抑制情况。结果：Cisplatin 剂量依赖性地抑制 A549、SKMES-1、MOR 和 H460 细胞生长，IC50 分别为 1.58 µM、4.09 µM、6.39 µM 和 5.72 µM。[1] 方法：人乳腺癌 MCF-7 和 MDA-MB-231 用 Cisplatin (2-10 μg/mL) 处理 48 h，使用 Western Blot 方法检测靶点蛋白表达水平。结果：Cisplatin 剂量依赖性诱导 MCF-7 和 MDA-MB-231 细胞中凋亡相关蛋白 cleaved-caspase 3 和 cleaved-PARP 的水平增加。[2] 方法：间皮瘤细胞 JU77、LO68 和 ONE58 用 Cisplatin (5-100 μg/mL) 处理 24 h，使用 JC-1 染料检测线粒体膜电位 (MMP)。结果：Cisplatin 剂量依赖性降低 JU77、LO68 和 ONE58 细胞的 MMP，抑制线粒体功能。[3]
体内活性	方法：为检测体内抗肿瘤活性，将 Cisplatin (5 mg/kg/6 天) 和 Chloroquine (13 mg/kg/天) 腹腔注射给携带咽下鳞状细胞癌肿瘤 (HSCC) FaDu 的 BALB/c nude 小鼠，持续十八天。结果：Cisplatin 治疗显著抑制 HSCC 肿瘤生长，Chloroquine 抑制自噬并增加 Cisplatin 诱导的细胞凋亡，从而增强了 Cisplatin 的疗效，导致小鼠的肿瘤生长减少和生存期延长。[4] 方法：为减轻 Cisplatin 治疗引起的肾毒性，将 Cisplatin (3-6 mg/kg/3天) 腹腔注射和 Cilastatin (100 mg/kg/天) 皮下注射给人肺腺癌肿瘤 A549 的 BALB/c 小鼠，持续七天。结果：Cilastatin 可以在不影响 Cisplatin 抗肿瘤作用的情况下减少其诱导的肾毒性。[5]
细胞实验	Rabbit renal proximal tubules were isolated using the iron oxide perfusion method and grown in 35-mm tissue culture dishes under improved conditions as described previously. The cell culture medium was a 1:1 mixture of Dulbecco's modified Eagle's medium/Ham's F-12 (without D-glucose, phenol red, or sodium pyruvate) supplemented with 15 mM HEPES buffer, 2.5 mM L-glutamine, 1 μM pyridoxine HCl, 15 mM sodium bicarbonate, and 6 mM lactate. Hydrocortisone (50 nM), selenium (5 ng/ml), human transferrin (5 μg/ml), bovine insulin (10 nM), and L-ascorbic acid-2-phosphate (50 μM) were added to fresh culture medium immediately before daily media change. In general, confluent RPTCs were treated with inhibitors or diluent control [typically DMSO at 0.1% (v/v)] for 30 min before treatment with cisplatin. Aliquots of RPTCs were used for various assays as detailed below [1].
动物实验	Mice were divided randomly into three groups (Control, Cisplatin and Cisplatin+HemoHIM), and each group consisted of twenty mice. B16F0 melanoma (5 × 10^5 cells/mouse) was inoculated into subcutaneous femoral left region of mice at 3 days before an initial injection of cisplatin. Cisplatin was injected intraperitoneally at 4 mg/kg body weight (B.W.) on day 0, 7 and 14 (total three injections). Experimental group was intubated with HemoHIM at a final concentration of 100 mg/kgB.W. by everyday from day -1 to day 16, while the control group received only water. On day 17 after initial injection of cisplatin, all mice of each group were experimented, respectively, to evaluate tumor weight or tumor size. The tumor size was calculated as follows: tumor size = ab^2/2, where a and b are the larger and smaller diameters, respectively [3].
别名	顺铂, cis-Diaminodichloroplatinum, CDDP

化学信息

分子量	300.04
分子式	Cl₂H₆N₂Pt
CAS No.	15663-27-1
Smiles	N[Pt](Cl)(Cl)N
密度	3.7

储存&溶解度

存储

keep away from direct sunlight | Powder: -20°C for 3 years | Shipping with blue ice/Shipping at ambient temperature.

溶解度信息

H2O: 1 mg/mL (3.3 mM), Heating to 50℃ is recommended.（DMSO can inactivate Cisplatin's activity.)

DMF: 20 mg/mL (66.66 mM), Sonication is recommended.

溶液配制表

H2O/DMF

	1mg	5mg	10mg	50mg
1 mM	3.3329 mL	16.6644 mL	33.3289 mL	166.6444 mL

DMF

	1mg	5mg	10mg	50mg
5 mM	0.6666 mL	3.3329 mL	6.6658 mL	33.3289 mL
10 mM	0.3333 mL	1.6664 mL	3.3329 mL	16.6644 mL
20 mM	0.1666 mL	0.8332 mL	1.6664 mL	8.3322 mL
50 mM	0.0667 mL	0.3333 mL	0.6666 mL	3.3329 mL

体内实验配液计算器

请在以下方框中输入您的动物实验信息后点击计算，可以得到母液配置方法和体内配方的制备方法：

比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， TargetMol | Animal experiments

一共给药动物 10 只 ，您使用的配方为 5% TargetMol | reagent

DMSO+ 30%PEG300+ 5%Tween 80 + 60%Saline/PBS/ddH₂O，那么您的工作液浓度为 2 mg/mL 。

母液配置方法: 2 mg 药物溶于 50 μLDMSO TargetMol | reagent

（母液浓度为 40 mg/mL ）, 如您需要配置的浓度超过该产品的溶解度，请先与我们联系。

体内配方的制备方法: 取 50μLDMSO TargetMol | reagent

母液，添加 300 μLPEG300 TargetMol | reagent

混匀澄清，再加 50μLTween 80, 混匀澄清，再加 600μLSaline/PBS/ddH₂O TargetMol | reagent

混匀澄清

以上为“体内实验配液计算器”的使用方法举例，并不是具体某个化合物的推荐配制方式，请根据您的实验动物和给药方式选择适当的溶解方案。

Cisplatin

Cisplatin

产品介绍

化合物与蛋白结合的复合物

计算器

体内实验配液计算器

剂量转换

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