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c-Kit Protein, Human, Recombinant (His)

c-Kit Protein, Human, Recombinant (His)

产品编号 TMPY-01935
别名: PBT, CD117, v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, SCFR, C-Kit

c-Kit Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 56.7 kDa and the accession number is P10721-2.

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c-Kit Protein, Human, Recombinant (His)
规格 价格/CNY 货期 数量
100 μg ¥ 3,820 现货
200 μg ¥ 6,750 5日内发货
500 μg ¥ 14,280 5日内发货
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产品目录号及名称: c-Kit Protein, Human, Recombinant (His) (TMPY-01935)
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生物活性
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参考文献
生物活性 Measured by its binding ability in a functional ELISA. Immobilized human KIT at 2 μg/mL (100 μl/well) can bind biotinylated Human KITL , The EC50 of biotinylated Human KITL is 2.0-9.0 ng/mL.

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产品描述 c-Kit Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 56.7 kDa and the accession number is P10721-2.
种属 Human
表达系统 HEK293 Cells
标签 C-His
蛋白编号 P10721-2
别名 PBT, CD117, v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, SCFR, C-Kit
蛋白构建 The Human KIT isoform 2 (P10721-2) extracellular domain (Met 1-Thr 516) was expressed, with a polyhistidine tag at the C-terminus.
蛋白纯度 ≥ 97 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC.

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分子量 56.7 kDa (predicted)
内毒素 < 1.0 EU/μg of the protein as determined by the LAL method.
缓冲液 Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
复溶方法 A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
存储

It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

运输方式

In general, Lyophilized powders are shipping with blue ice.

研究背景 C-Kit is a type 3 transmembrane receptor for MGF (mast cell growth factor, also known as stem cell factor). c-Kit contains 5 Ig-like C2-type (immunoglobulin-like) domains and 1 protein kinase domain. It belongs to the protein kinase superfamily, tyr protein kinase family, and CSF-1/PDGF receptor subfamily. C-Kit has tyrosine-protein kinase activity. Binding of the ligands leads to the autophosphorylation of KIT and its association with substrates such as phosphatidylinositol 3-kinase. Antibodies to c-Kit are widely used in immunohistochemistry to help distinguish particular types of tumor in histological tissue sections. It is used primarily in the diagnosis of GISTs. In GISTs, c-Kit staining is typically cytoplasmic, with stronger accentuation along the cell membranes. C-Kit antibodies can also be used in the diagnosis of mast cell tumors and in distinguishing seminomas from embryonal carcinomas. Mutations in the c-Kit gene are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous leukemia, and piebaldism. Defects in KIT are a cause of acute myelogenous leukemia (AML). AML is a malignant disease in which hematopoietic precursors are arrested in an early stage of development. Note=Somatic mutations that lead to constitutive activation of KIT are detected in AML patients.Cancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy

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Keywords

c-Kit Protein, Human, Recombinant (His) PBT CD117 CD 117 v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog SCFR C-Kit CD-117 recombinant recombinant-proteins proteins protein

 

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