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c-Kit Protein, Mouse, Recombinant (His)

产品编号 TMPY-01802

别名: SCO5, SCO1, Bs, Gsfsco1, v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, W, Ssm, SOW3, Fdc, CD117, Gsfsow3, Gsfsco5, c-KIT, Tr-kit

c-Kit Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 57 kDa and the accession number is P05532-1.

TargetMol的所有产品和服务仅用于科学研究，不能被用于人体，我们也不向个人提供产品和服务。

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c-Kit Protein, Mouse, Recombinant (His)

规格	价格/CNY		货期	数量
100 μg	￥ 3,820		现货
200 μg	￥ 6,750		5日内发货
500 μg	￥ 14,280		5日内发货

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参考文献

生物活性	Measured by its binding ability in a functional ELISA. Immobilized mouse KIT at 2 μg/ml (100 μl/well) can bind biotinylated mouse KITL with a linear range of 1.28-6.4 ng/ml.
产品描述	c-Kit Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 57 kDa and the accession number is P05532-1.

种属	Mouse
表达系统	HEK293 Cells
标签	C-His
蛋白编号	P05532-1
别名	SCO5, SCO1, Bs, Gsfsco1, v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, W, Ssm, SOW3, Fdc, CD117, Gsfsow3, Gsfsco5, c-KIT, Tr-kit
蛋白构建	The Mouse KIT isoform 1 (NP_001116205.1) extracellular domain (Met 1-Thr 523) was fused with a polyhistidine tag at the C-terminus.
蛋白纯度	≥ 97 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC.
分子量	57 kDa (predicted)

内毒素	< 1.0 EU/μg of the protein as determined by the LAL method.
缓冲液	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
复溶方法	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
存储	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式	In general, Lyophilized powders are shipping with blue ice.
研究背景	C-Kit is a type 3 transmembrane receptor for MGF (mast cell growth factor, also known as stem cell factor). c-Kit contains 5 Ig-like C2-type (immunoglobulin-like) domains and 1 protein kinase domain. It belongs to the protein kinase superfamily, tyr protein kinase family, and CSF-1/PDGF receptor subfamily. C-Kit has tyrosine-protein kinase activity. Binding of the ligands leads to the autophosphorylation of KIT and its association with substrates such as phosphatidylinositol 3-kinase. Antibodies to c-Kit are widely used in immunohistochemistry to help distinguish particular types of tumor in histological tissue sections. It is used primarily in the diagnosis of GISTs. In GISTs, c-Kit staining is typically cytoplasmic, with stronger accentuation along the cell membranes. C-Kit antibodies can also be used in the diagnosis of mast cell tumors and in distinguishing seminomas from embryonal carcinomas. Mutations in the c-Kit gene are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous leukemia, and piebaldism. Defects in KIT are a cause of acute myelogenous leukemia (AML). AML is a malignant disease in which hematopoietic precursors are arrested in an early stage of development. Note=Somatic mutations that lead to constitutive activation of KIT are detected in AML patients.Cancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy

参考文献

1. Andre C, et al. (1997) Sequence analysis of two genomic regions containing the KIT and the FMS receptor tyrosine kinase genes. Genomics. 39(2):216-26. 2. Yarden Y, et al. (1987) Human proto-oncogene c-kit: a new cell surface receptor tyrosine kinase for an unidentified ligand. EMBO J. 6(11):3341-51. 3. Leong KG, et al. (2008) Generation of a prostate from a single adult stem cell. Nature. 456(7223): 804-8. 4. Edling CE, et al. (2007) c-Kit--a hematopoietic cell essential receptor tyrosine kinase. Int J Biochem Cell Biol. 39(11):1995-8. 5. McIntyre A, et al. (2005) Amplification and overexpression of the KIT gene is associated with progression in the seminoma subtype of testicular germ cell tumors of adolescents and adults. Cancer Res. 65(18):8085-9.

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Keywords

c-Kit Protein, Mouse, Recombinant (His) SCO5 SCO1 CD-117 Bs Gsfsco1 v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog CD 117 W SCO 5 Ssm SCO-5 SCO 1 SOW3 SCO-1 Fdc SOW-3 SOW 3 CD117 Gsfsow3 Gsfsco5 c-KIT Tr-kit recombinant recombinant-proteins proteins protein

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