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Phenol Red

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纯度: 98.95%

货号 T0347Cas号 143-74-8

别名 酚红, Sulphental, Sulfonphthal, Fenolipuna

Phenol Red (Sulfonphthal) 是一种pH 指示染料,当出现阳性反应时,会发生由粉红色到黄色的明显颜色变化。

Phenol Red
其他形式的 “Phenol Red”:

Phenol Red

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Rating icon 很棒

纯度: 98.95%

货号 T0347 别名 酚红, Sulphental, Sulfonphthal, FenolipunaCas号 143-74-8

Phenol Red (Sulfonphthal) 是一种pH 指示染料,当出现阳性反应时,会发生由粉红色到黄色的明显颜色变化。

规格价格库存数量
200 mg
¥ 99
现货
1 mL x 10 mM (in DMSO)
¥ 195
现货
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纯度: 98.95%
颜色: 红色
性状: Solid
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产品介绍


Phenol Red AI Summary
Phenol Red exhibits a broad range of bioactivities targeting various biological pathways and processes. Notably, it significantly inhibits amyloid beta-42 fibrillation by 132.0% and has an IC50 value of 426250.0 nM for inhibiting amyloid beta-42 oligomerization, as measured by ThT fluorescence analysis and ELISA respectively, indicating its potential therapeutic properties for amyloid beta-related conditions. Moreover, it demonstrates activity against SARS-CoV-2, including a 43.97% inhibition of virus-induced cytotoxicity in Caco-2 cells at 10 µM concentration after 48 hours. Phenol Red also inhibits the SARS-CoV-2 3CL-Pro protease by 7.074% at a 20 µM concentration, and shows moderate antiviral activity in HRCE cells with a hit score of 0.07808. Additionally, Phenol Red inhibits several key molecular interactions and pathways, including the Menin-MLL interaction, Lamin A splicing, Hypoxia Response Element signaling (both as an agonist and antagonist), and the Anthrax Lethal Toxin internalization process. It acts as an inhibitor for Cytochrome P450 2C9 and Histone Lysine Methyltransferase G9a, with activity in cytochrome panel assays involving p450-cyp2c9 and p450-cyp1a2. In cancer cell line growth inhibition assays, it shows a GI50 value of 100000.0 nM across various human tumor cell lines. In terms of metabolic enzyme activity, Phenol Red exhibits activity with the human recombinant UGT1A9 enzyme but shows no activity in UGT1A1 and UGT1A4 assays. It also demonstrates a permeability coefficient (Papp) of 0.35 x 10^-6 cm/s in human Caco-2 cells, suggesting potential bioavailability. Finally, Phenol Red shows inhibitory activity against human HDAC6, achieving 13.9% inhibition with a commercial peptide substrate and 11.16% with a custom peptide substrate. Its diverse bioactivities suggest potential therapeutic applications in neurodegenerative diseases, antiviral treatments, oncology, and various other conditions..
Note: Summary generated by AI. Data source: ChEMBL
生物活性
产品描述
Phenol Red (Sulfonphthal) is a pH indicator, red dye, and diagnostic aid for measurement of renal function. It is used also for researches of the gastrointestinal and other systems.
体外活性

使用方法
一、溶液配置

  1. 母液配置:将Phenol溶解于适当的溶剂中,henol可溶于碱性溶液,如氢氧化钠或碳酸钠溶液,常用浓度为 5 mg/mL 或 20 mg/mL。
    2.工作液配置:Phenol的常用终浓度为 15–20 mg/L。
    注意事项:请过滤灭菌:使用 0.22 µm 滤膜过滤,以确保溶液无菌。将溶液分装于避光容器中,4°C 或者-80℃保存。
    二、操作步骤
    指示功能:Phenol在 pH 6.8–8.2 范围内呈现颜色变化,可指示培养基的 pH 状态。
  2. 在 pH 测定中的应用
    测定范围:Phenol适用于 pH 6.5–8.4 的测定。
    1).样品准备:取 10 mL 待测液于比色杯中。
    2)添加指示剂:加入适量酚红溶液(如 6 滴),混匀。
    3)比色测定:使用比色计在适当波长下测定吸光度,或与标准比色卡比较,确定 pH 值。

以上信息均来源于已发表文献,具体实验方案请根据实际研究需求进行适当调整。

别名酚红, Sulphental, Sulfonphthal, Fenolipuna
化学信息
分子量354.38
分子式C19H14O5S
CAS No.143-74-8
SmilesOC1=CC=C(C=C1)C1(OS(=O)(=O)C2=C1C=CC=C2)C1=CC=C(O)C=C1
密度1.477 g/cm3
储存&溶解度
存储keep away from direct sunlight,store under nitrogen | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
溶解度信息
DMSO: 247 mg/mL (696.99 mM), Sonication is recommended.
体内实验配方
10% DMSO+40% PEG300+5% Tween-80+45% Saline: 3.3 mg/mL (9.31 mM), Sonication is recommended.
请按顺序添加溶剂,在添加下一种溶剂之前,尽可能使溶液澄清。如有必要,可通过加热、超声、涡旋处理进行溶解。工作液建议现配现用。以上配方仅供参考,体内配方并不是绝对的,请根据不同情况进行调整。
溶液配制表
DMSO
1mg5mg10mg50mg
1 mM2.8218 mL14.1091 mL28.2183 mL141.0915 mL
5 mM0.5644 mL2.8218 mL5.6437 mL28.2183 mL
10 mM0.2822 mL1.4109 mL2.8218 mL14.1091 mL
20 mM0.1411 mL0.7055 mL1.4109 mL7.0546 mL
50 mM0.0564 mL0.2822 mL0.5644 mL2.8218 mL
100 mM0.0282 mL0.1411 mL0.2822 mL1.4109 mL

计算器

  • 摩尔浓度 计算器
  • 稀释 计算器
  • 配液 计算器
  • 分子量 计算器

体内实验配液计算器

请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法:
比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL, 一共给药动物10只,您使用的配方为 10% DMSO + 40% PEG300 + 5% Tween 80 + 45% Saline / PBS / ddH2O, 那么您的工作液浓度为2 mg/mL
母液配置方法:2 mg 药物溶于 100 μL DMSO ( 母液浓度为 20 mg/mL ), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:100 μL DMSO 母液, 添加 400 μL PEG300 混匀澄清, 再加 50 μL Tween 80, 混匀澄清, 再加 450 μL Saline / PBS / ddH2O 混匀澄清
以上为“体内实验配液计算器”的使用方法举例,并不是具体某个化合物的推荐配制方式,请根据您的实验动物和给药方式选择适当的溶解方案。
方案所需的各类助溶剂如: DMSOPEG300PEG400Tween 80SBE-β-CD玉米油等, 均可在TargetMol网站点击购买。
1 请输入动物实验的基本信息
mg/kg
g
μL
2 请输入动物体内配方组成,不同的产品配方组成不同,如有配方需求,可先联系我们提供正确的体内配方。
% DMSO
%
% Tween 80
% Saline/PBS/ddH2O

剂量转换

对于不同动物的给药剂量换算,您也可以参考 更多

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