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Anti-Galectin-1 Antibody (5G581)
还可以产品编号 TMAY-00564
Anti-Galectin-1 Antibody (5G581) 是一种 Mouse 抗体，靶向 Galectin-1。Anti-Galectin-1 Antibody (5G581) 可用于 WB,IP。
Anti-Galectin-1 Antibody (5G581)
Anti-Galectin-1 Antibody (5G581)
还可以产品编号 TMAY-00564
Anti-Galectin-1 Antibody (5G581) 是一种 Mouse 抗体，靶向 Galectin-1。Anti-Galectin-1 Antibody (5G581) 可用于 WB,IP。
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产品介绍
生物活性
偶联与修饰
抗原信息
| 产品描述 | Anti-Galectin-1 Antibody (5G581) is a Mouse antibody targeting Galectin-1. Anti-Galectin-1 Antibody (5G581) can be used in WB,IP. |
| Ig Type | Monoclonal Mouse IgG1 |
| 克隆号 | 5G581 |
| 交叉反应 | Human |
| 验证活性 | 1. Anti-Galectin1 mouse monoclonal antibody at 1:500 -Lane A: Hela Whole Cell Lysate. -Lane B: A431 Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution. -Developed using the Odyssey technique. -Performed under reducing conditions. 2. Galectin1 was immunoprecipitated using: -Lane A:0.5 mg Hela Whole Cell Lysate -0.5 µL anti-Galectin1 mouse monoclonal antibody and 15 μl of 50 % Protein G agarose. -Primary antibody: -Anti-Galectin1 mouse monoclonal antibody, at 1:250 dilution. -Secondary antibody: -Dylight 800-labeled antibody to Mouse IgG (H+L), at 1:7500 dilution. -Developed using the odyssey technique. -Performed under reducing conditions. -Predicted band size: 15 kDa. -Observed band size: 15 kDa |
| 应用 | WB,IP |
| 推荐剂量 | WB: 1:500-1:1000; IP: 0.2-1 μL/mg of lysate |
| 抗体种类 | Monoclonal |
| 宿主来源 | Mouse |
| 构建方式 | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human Galectin-1 / LGALS1 (rh Galectin-1 / LGALS1; TMPY-00976; NP_002296.1; Met1-Asp135). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
| 纯化方式 | Protein A |
| 性状 | Liquid |
| 缓冲液 | 0.2 μm filtered solution in PBS |
| 研究背景 | Galectin-1 (Gal-1, GAL1), is a member of the galectins, a family of animal lectins ranging from Caenorhabditis elegans to humans, which is defined by their affinity for beta-galactosides and by significant sequence similarity in the carbohydrate-binding site. It is a homodimer with a subunit molecular mass of 14.5 kDa, which contains six cysteine residues per subunit. The cysteine residues should be in a free state to maintain a molecular structure that is capable of showing lectin activity. This endogenous lectin widely expressed at sites of inflammation and tumor growth has been postulated as an attractive immunosuppressive agent to restore immune cell tolerance and homeostasis in autoimmune and inflammatory settings. On the other hand, galectin-1 contributes to different steps of tumor progression including cell adhesion, migration, and tumor-immune escape, suggesting that blockade of galectin-1 might result in therapeutic benefits in cancer. Several potential glycoprotein ligands for galectin-1 have been identified, including lysosome-associated membrane glycoproteins and fibronectin, laminin, as well as T-cell glycoproteins CD43 and CD45. Evidence points to Gal-1 and its ligands as one of the master regulators of such immune responses as T-cell homeostasis and survival, T-cell immune disorders, inflammation, and allergies as well as host-pathogen interactions. |
| 偶联 | Unconjugated |
| 免疫原 | Recombinant Human Galectin-1 / LGALS1 protein (TMPY-00976) |
| 抗原种属 | Human |
存储&运输
| 储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| 运输方式 | Shipping with blue ice. |
SCI 文献
计算器
比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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