Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Vincristine sulfate (Leurocristine sulfate) 是一种抗肿瘤长春花生物碱。在细胞周期的 S 期不可逆地与微管(Ki: 85 nM) 和纺锤体蛋白结合,并干扰有丝分裂纺锤体的形成。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
20 mg | ¥ 866 | 现货 | ||
1 mL * 10 mM (in DMSO) | ¥ 511 | 现货 |
产品描述 | Vincristine sulfate (Leurocristine sulfate) binds irreversibly to microtubules and spindle proteins in S phase and interferes the formation of the mitotic spindle. |
靶点活性 | Microtubule:85 nM (Ki, cell free) |
体外活性 | Vincristine 抑制微管蛋白添加(Ki,0.085µg/mL)。在L细胞中,Vincristine 在40纳摩尔浓度下引起约25%的生长抑制[1]。Vincristine 抑制SH-SY5Y细胞增殖呈时间和剂量依赖性(IC50:0.1µg/mL)。0.1µg/mL的VCR诱导细胞进入有丝分裂阻塞和凋亡,促进caspase-3和-9以及cyclin B的表达,同时减少cyclin D在6、12、18和24小时的表达[2]。在体外研究了Vincristine 在0.05mM浓度下对猫迷走神经轴突超微结构的影响。0.1mM的Vincristine 引起微管结构破坏、顺晶结构出现以及神经原纤维的增加[3]。 |
细胞实验 | SH-SY5Y cells at a logarithmic phase were seeded in 96-well plates (at 2x10^6/l) and incubated for 12 h until cells formed a monolayer. Wells were randomly chosen for treatment groups and a control group. For the treatment groups, cells were incubated with 200 μl of cell culture medium containing 0.001, 0.01, 0.1, 1 or 10 μM of VCR. In the control group, cells were grown in 200 μl cell culture medium only. Cells were incubated for another 24, 48 and 72 h and then 20 μl of 5 g/l MTT (0.1 mg/l final concentration) was added to each well. After 4 h of incubation, the cell culture supernatant was removed, 150 μl of DMSO was added to each well and the plate was shaken for 10 min. The absorbance of each well was detected at 490 nm (A value) on an ELISA plate reader. The growth inhibition rate of VCR-treated cells was calculated as: Growth inhibition rate % = [(average A value of control group - average A value of VCR-treated group)/average A value of control group] x 100%. This experiment was performed in triplicates [2]. |
动物实验 | Vincristine (1?mg/ml) was diluted in saline and administered i.v. to wild-type and Mdr1ab/Mrp1 TKO mice, aged 10–14 weeks, at dose levels ranging between 0.125 and 4?mg/kg. Animals were monitored daily and killed when they lost more than 20% of their initial body weight. The MTD was defined as one dose step below the dose where more than one animal in that group had to be killed. Necropsies were performed in wild-type and Mdr1ab/Mrp1 TKO mice receiving vincristine at or near the MTD and killed 2 days later [5]. |
别名 | Leurocristine, 22-Oxovincaleukoblastine sulfate, Leurocristine sulfate, 硫酸长春新碱 |
分子量 | 923.04 |
分子式 | C46H58N4O14S |
CAS No. | 2068-78-2 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 50 mg/mL (54.17 mM)
Ethanol: < 1 mg/mL (insoluble or slightly soluble)
H2O: 92.3 mg/mL(100 mM)
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
DMSO / H2O | 1 mM | 1.0834 mL | 5.4169 mL | 10.8338 mL | 27.0844 mL |
5 mM | 0.2167 mL | 1.0834 mL | 2.1668 mL | 5.4169 mL | |
10 mM | 0.1083 mL | 0.5417 mL | 1.0834 mL | 2.7084 mL | |
20 mM | 0.0542 mL | 0.2708 mL | 0.5417 mL | 1.3542 mL | |
50 mM | 0.0217 mL | 0.1083 mL | 0.2167 mL | 0.5417 mL | |
H2O | 100 mM | 0.0108 mL | 0.0542 mL | 0.1083 mL | 0.2708 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
Vincristine sulfate 2068-78-2 Apoptosis Cytoskeletal Signaling Microtubule Associated Inhibitor Microtubule/Tubulin NSC-67574 Leurocristine 22-Oxovincaleukoblastine sulfate Vincristine Vincristine Sulfate 22-Oxovincaleukoblastine NSC67574 Leurocristine Sulfate Leurocristine sulfate inhibit NSC 67574 硫酸长春新碱 22-Oxovincaleukoblastine Sulfate inhibitor