首页 工具
登录
购物车
3-Deazaadenosine hydrochloride

3-Deazaadenosine hydrochloride

产品编号 T10111   CAS 86583-19-9

3-Deazaadenosine hydrochloride 是 S-腺苷高半胱氨酸水解酶的抑制剂,Ki 值为 3.9 µM。 它具有抗炎、抗增殖和抗 HIV 活性。

TargetMol的所有产品和服务仅用于科学研究,不能被用于人体,我们也不向个人提供产品和服务。
3-Deazaadenosine hydrochloride Chemical Structure
3-Deazaadenosine hydrochloride, CAS 86583-19-9
规格 价格/CNY 货期 数量
1 mg ¥ 1,370 现货
5 mg ¥ 3,120 现货
10 mg ¥ 4,580 现货
25 mg ¥ 7,270 现货
50 mg ¥ 9,780 现货
100 mg ¥ 13,200 现货
1 mL * 10 mM (in DMSO) ¥ 1,990 现货
其他形式的 3-Deazaadenosine hydrochloride:
产品目录号及名称: 3-Deazaadenosine hydrochloride (T10111)
点击图片重新获取验证码
选择批次  
纯度: 99.99%
更多批次查询请联系客服
生物活性
化学信息
存储 & 溶解度
参考文献
产品描述 3-Deazaadenosine hydrochloride is an inhibitor of S-adenosylhomocysteine hydrolase (Ki: 3.9 μM). It has anti-inflammatory, anti-proliferative, and anti-HIV activity.
靶点活性 HIV-1, A018 isolate:0.20 µM , HIV-1, A012 isolate:0.15, S-adenosylhomocysteine hydrolase:(ki)3.9 µM
体外活性 3-Deazaadenosine shows the anti-HIV effect and inhibits p24 antigen in peripheral blood mononuclear (PBMCs) cells infected with HIV-1 isolates (A012 and A018, IC50s: 0.15 and 0.20 μM) [1]. 3-Deazaadenosine (1-100 μM) inhibits LPS-induced expression of TNF-α mRNA, increases DNA binding activity of NF-κB, and causes proteolytic degradation of IκBα, but Not IκBβ in RAW 264.7 cells. 3-Deazaadenosine (100 μM) enhances the nuclear translocation of NF-κB but blocks LPS-induced NF-κB transcriptional activity, and such inhibition is augmented by the addition of homocysteine [2]. 3-Deazaadenosine (50, 100 μM) dose-dependently inhibits the phosphorylation of Raf and ERK, protein-dependent kinase 1, protein kinase B (Akt), and forkhead transcription factor FoxO1a. 3-Deazaadenosine (50 μM) suppresses vascular smooth muscle cell (VSMC) proliferation via interfering with Ras signaling [3].
细胞实验 The HIV-1 strains A012 and A018 are used in the assay. Inhibition of p24 antigen is measured. Briefly, PHA-stimulated peripheral blood mononuclear (PBMCs) are incubated with either HIV-1 strain for 1 h at 37°C at 200-fold the 50% tissue culture infectious dose (TCID50) of the virus stock per 2 × 10^5 PBMC cells. The TCID50 is defined as the amount of virus stock at which 50% of the inoculated wells are positive. Cells are then grown in microtiter plates with different drug concentrations at 2 × 10^5 cells per well. On day 4, cells are resuspended and split 1:3 with fresh media and 3-Deazaadenosine. Supernatant p24 antigen is determined on day 7 by ELISA [1].
分子量 302.71
分子式 C11H15ClN4O4
CAS No. 86583-19-9

存储

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

溶解度

DMSO: 40.67 mg/mL(134.35 mM)

H2O: 20 mg/mL

溶液配制表

可选溶剂 浓度 体积 质量 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.3035 mL 16.5175 mL 33.0349 mL 82.5873 mL
5 mM 0.6607 mL 3.3035 mL 6.607 mL 16.5175 mL
10 mM 0.3303 mL 1.6517 mL 3.3035 mL 8.2587 mL
20 mM 0.1652 mL 0.8259 mL 1.6517 mL 4.1294 mL
50 mM 0.0661 mL 0.3303 mL 0.6607 mL 1.6517 mL
100 mM 0.033 mL 0.1652 mL 0.3303 mL 0.8259 mL

计算器

摩尔浓度计算器
稀释计算器
配液计算器
分子量计算器
=
X
X
X
=
X
=
/
g/mol

输入分子式,点击计算,可计算出产品的分子量。

参考文献

1. Gordon RK, et al. Anti-HIV-1 activity of 3-deaza-adenosine analogs. Inhibition of S-adenosylhomocysteine hydrolase and nucleotide congeners. Eur J Biochem. 2003 Sep;270(17):3507-17. 2. Jeong SY, et al. 3-deazaadenosine, a S-adenosylhomocysteine hydrolase inhibitor, has dual effects on NF-kappaB regulation. Inhibition of NF-kappaB transcriptional activity and promotion of IkappaBalpha degradation. J Biol Chem. 1999 Jul 2;274(27):18981-8. 3. Sedding DG, et al. 3-Deazaadenosine prevents smooth muscle cell proliferation and neointima formation by interfering with Ras signaling. Circ Res. 2009 May 22;104(10):1192-200.
Helichrysetin NBD-556 HIV-1 Rev (34-50) Salaspermic acid Bellidifolin Bz-RS-ISer(3-Ph)-Ome Dolutegravir sodium Cytochalasin A

相关化合物库

该产品包含在如下化合物库中:
毒性化合物库 NO PAINS 化合物库 抗病毒库 ReFRAME 相关化合物库 核苷类化合物库 已知活性化合物库 抑制剂库 抗纤维化化合物库 细胞凋亡化合物库 抗衰老化合物库

剂量换算

对于不同动物的给药剂量换算,您也可以参考 更多...

体内实验配液计算器

请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。

母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。

体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。

第一步:请输入动物实验的基本信息
剂量
mg/kg
每只动物体重
g
给药体积
μL
动物数量
第二步:请输入动物体内配方组成,不同的产品配方组成不同,如有配方需求,可先联系我们提供正确的体内配方。
% DMSO
%
% Tween 80
% ddH2O
计算 重置

技术支持

您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。

Keywords

3-Deazaadenosine hydrochloride 86583-19-9 Microbiology/Virology Others Proteases/Proteasome HIV Protease HIV 3-Deazaadenosine Human immunodeficiency virus Inhibitor 3-Deazaadenosine Hydrochloride 3 Deazaadenosine hydrochloride 3Deazaadenosine hydrochloride inhibit inhibitor

 

陶术
生物
TargetMol®中国区唯一合作伙伴
点击进入陶术生物官网陶术生物
联系我们
400-820-0310

上海市静安区江场三路238号8楼