Powder: -20°C for 3 years | In solvent: -80°C for 1 year
3-Deazaadenosine hydrochloride 是 S-腺苷高半胱氨酸水解酶的抑制剂,Ki 值为 3.9 µM。 它具有抗炎、抗增殖和抗 HIV 活性。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
1 mg | ¥ 1,370 | 现货 | ||
5 mg | ¥ 3,120 | 现货 | ||
10 mg | ¥ 4,580 | 现货 | ||
25 mg | ¥ 7,270 | 现货 | ||
50 mg | ¥ 9,780 | 现货 | ||
100 mg | ¥ 13,200 | 现货 | ||
1 mL * 10 mM (in DMSO) | ¥ 1,990 | 现货 |
产品描述 | 3-Deazaadenosine hydrochloride is an inhibitor of S-adenosylhomocysteine hydrolase (Ki: 3.9 μM). It has anti-inflammatory, anti-proliferative, and anti-HIV activity. |
靶点活性 | HIV-1, A018 isolate:0.20 µM , HIV-1, A012 isolate:0.15, S-adenosylhomocysteine hydrolase:(ki)3.9 µM |
体外活性 | 3-Deazaadenosine shows the anti-HIV effect and inhibits p24 antigen in peripheral blood mononuclear (PBMCs) cells infected with HIV-1 isolates (A012 and A018, IC50s: 0.15 and 0.20 μM) [1]. 3-Deazaadenosine (1-100 μM) inhibits LPS-induced expression of TNF-α mRNA, increases DNA binding activity of NF-κB, and causes proteolytic degradation of IκBα, but Not IκBβ in RAW 264.7 cells. 3-Deazaadenosine (100 μM) enhances the nuclear translocation of NF-κB but blocks LPS-induced NF-κB transcriptional activity, and such inhibition is augmented by the addition of homocysteine [2]. 3-Deazaadenosine (50, 100 μM) dose-dependently inhibits the phosphorylation of Raf and ERK, protein-dependent kinase 1, protein kinase B (Akt), and forkhead transcription factor FoxO1a. 3-Deazaadenosine (50 μM) suppresses vascular smooth muscle cell (VSMC) proliferation via interfering with Ras signaling [3]. |
细胞实验 | The HIV-1 strains A012 and A018 are used in the assay. Inhibition of p24 antigen is measured. Briefly, PHA-stimulated peripheral blood mononuclear (PBMCs) are incubated with either HIV-1 strain for 1 h at 37°C at 200-fold the 50% tissue culture infectious dose (TCID50) of the virus stock per 2 × 10^5 PBMC cells. The TCID50 is defined as the amount of virus stock at which 50% of the inoculated wells are positive. Cells are then grown in microtiter plates with different drug concentrations at 2 × 10^5 cells per well. On day 4, cells are resuspended and split 1:3 with fresh media and 3-Deazaadenosine. Supernatant p24 antigen is determined on day 7 by ELISA [1]. |
分子量 | 302.71 |
分子式 | C11H15ClN4O4 |
CAS No. | 86583-19-9 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 40.67 mg/mL(134.35 mM)
H2O: 20 mg/mL
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
DMSO | 1 mM | 3.3035 mL | 16.5175 mL | 33.0349 mL | 82.5873 mL |
5 mM | 0.6607 mL | 3.3035 mL | 6.607 mL | 16.5175 mL | |
10 mM | 0.3303 mL | 1.6517 mL | 3.3035 mL | 8.2587 mL | |
20 mM | 0.1652 mL | 0.8259 mL | 1.6517 mL | 4.1294 mL | |
50 mM | 0.0661 mL | 0.3303 mL | 0.6607 mL | 1.6517 mL | |
100 mM | 0.033 mL | 0.1652 mL | 0.3303 mL | 0.8259 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
3-Deazaadenosine hydrochloride 86583-19-9 Microbiology/Virology Others Proteases/Proteasome HIV Protease HIV 3-Deazaadenosine Human immunodeficiency virus Inhibitor 3-Deazaadenosine Hydrochloride 3 Deazaadenosine hydrochloride 3Deazaadenosine hydrochloride inhibit inhibitor