propidium iodide

Propidium Iodide (PI) 是一种可用于细胞染色的红色荧光染料，适用于荧光显微镜、共聚焦显微镜、流式细胞仪以及荧光计分析。在水溶液中，Propidium Iodide 的 Ex Em 为 493 636 nm；与核酸结合后，PI-核酸的 Ex Em 变为 535 617 nm，同时荧光信号增强 20-30 倍。

Serpin A1 is a prototype member of the Serpin superfamily of the serine protease inhibitors. As one of the most abundant proteinase inhibitors in the circulation, it is synthesized in hepatocytes, and to a lesser extent, in macrophages as well as intestinal epithelial cell lines and secreted as the abundant proteinase inhibitor in the circulation whose targets include elastase, plasmin, thrombin, trypsin, chymotrypsin, and plasminogen activator. Point mutations in the native SerpinA1 variants result in Serpin A1 deficiency, and consequently lead to several clinical complications such as pulmonary emphysema, juvenile hepatitis, cirrhosis, and hepatocellular carcinoma. For example, the Z variants (Glu342 to Lys) forms intracellular inclusion bodies, is not secreted, and leads to a severe SerpinA1 deficiency. Accordingly, Serpin A1 deficiency in circulation is associated with emphysema or liver disease.

Expression system: HEK293 Cells
Length: 1-418, Full Length
Activity: Enzyme Activity