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GPRC5D Protein-VLP, Cynomolgus, Recombinant

产品编号 TMPK-00528

Chimeric antigen receptor (CAR) T cells, a type of cell-based immunotherapy, have shown some promising results in multiple myeloma, a bone marrow cancer.The orphan G protein–coupled receptor, class C group 5 member D (GPRC5D), normally expressed only in the hair follicle, Using quantitative immunofluorescence, we determined that GPRC5D protein is expressed on CD138 MM cells from primary marrow samples with a distribution that was similar to, but independent of, BCMA. GPRC5D Protein-VLP, Cynomolgus, Recombinant is expressed in HEK293 mammalian cells. The predicted molecular weight is 35.10 kDa and the accession number is A0A2K5W6I7.

GPRC5D Protein-VLP, Cynomolgus, Recombinant

GPRC5D Protein-VLP, Cynomolgus, Recombinant

产品编号 TMPK-00528
Chimeric antigen receptor (CAR) T cells, a type of cell-based immunotherapy, have shown some promising results in multiple myeloma, a bone marrow cancer.The orphan G protein–coupled receptor, class C group 5 member D (GPRC5D), normally expressed only in the hair follicle, Using quantitative immunofluorescence, we determined that GPRC5D protein is expressed on CD138 MM cells from primary marrow samples with a distribution that was similar to, but independent of, BCMA. GPRC5D Protein-VLP, Cynomolgus, Recombinant is expressed in HEK293 mammalian cells. The predicted molecular weight is 35.10 kDa and the accession number is A0A2K5W6I7.
规格价格库存数量
100 μg
¥ 10,300
5日内发货
500 μg
¥ 41,500
5日内发货
1 mg
¥ 69,200
5日内发货
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实验操作小课堂
常见问题解答
Tween-20 可以用 Tween-80 替代吗?
不建议使用 Tween-20。相较于 Tween-20,Tween-80 的耐受性更好,Tween-80已被用作评估实验药物和毒物的行为影响的工具,且没有明显的副作用。 参考文献:Castro CA, Hogan JB, Benson KA, Shehata CW, Landauer MR. Behavioral effects of vehicles: DMSO, ethanol, Tween-20, Tween-80, and emulphor-620. Pharmacol Biochem Behav. 1995 Apr;50(4):521-6.
产品的溶解度是多少?
建议您先查询TargetMol官网、陶术官网,看该抑制剂的产品介绍,找到溶解度信息。如果没有相应信息,建议联系技术对该化合物的溶解度进行测定。
如何稀释工作液?
根据您的工作液浓度,计算母液所需要稀释的倍数和所需母液的体积。建议用水、生理盐水、PBS等溶剂进行稀释,如果是细胞实验,可以使用培养基。 取适当的溶剂缓慢加入到母液中,直到达到您的工作液浓度,可以通过涡旋或者移液枪轻轻吹打帮助混匀。 我们的化合物大部分是脂溶性的,所以在使用 PBS 或者培养基等稀释工作液的时候,会有沉淀析出,这些都是正常现象,大多时候可以通过超声完全溶解。 工作液建议现配现用!
抑制剂产品应如何储存?
粉末储存在 -20℃,可以存 3 年以上。 母液一般储存于 -80℃,可以存 1 年以上,建议多分装几管,避免反复冻融。 常用的存于 4℃,可放一周以上。
得到的杀死细胞的 IC50 与网页上 IC50 差距较大怎么办?
您所测试的IC50是指细胞的增殖抑制实验(半抑制率),细胞增殖抑制实验 IC50值,和网页上的靶点IC50其实是不同的意义。文献里和网页上 IC50其实往往是指对于靶点无细胞实验的抑制率,这样的实验一般是通过激酶或者蛋白纯化实验做出来的,nM 级别的浓度比较常见,但是细胞的增殖抑制实验 IC50 是指细胞的半致死率,同时牵涉到细胞的代谢以及渗透,一般浓度会高一些。 另外,同一个化合物对不同细胞模型的效果也是不同的,建议尝试增加孵育量、延长孵育时间。
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产品信息

生物活性
Immobilized Cynomolgus GPRC5D VLP (100μl/well) at 5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-GPRC5D Antibody, hFc Tag with the EC50 of 6.91μg/ml determined by ELISA.
产品描述
Chimeric antigen receptor (CAR) T cells, a type of cell-based immunotherapy, have shown some promising results in multiple myeloma, a bone marrow cancer.The orphan G protein–coupled receptor, class C group 5 member D (GPRC5D), normally expressed only in the hair follicle, Using quantitative immunofluorescence, we determined that GPRC5D protein is expressed on CD138 MM cells from primary marrow samples with a distribution that was similar to, but independent of, BCMA. GPRC5D Protein-VLP, Cynomolgus, Recombinant is expressed in HEK293 mammalian cells. The predicted molecular weight is 35.10 kDa and the accession number is A0A2K5W6I7.
种属
Cynomolgus
表达系统
HEK293 Cells
蛋白编号A0A2K5W6I7
别名
orphan G-protein coupled receptor,MGC129714,MGC129713,GPRC5D
蛋白构建
Met1-Cys300
蛋白纯度
> 95% as determined by HPLC
分子量35.10 kDa (predicted)
内毒素< 1 EU/μg by the LAL method.
缓冲液Supplied as 0.22 μm filtered solution in PBS (pH 7.4).Notice: If you need it for immunization, Do Not use any adjuvant.
存储
It is recommended to store the product under sterile conditions at -70°C or lower. Samples are stable for up to 12 months at -80°C. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式In general, Lyophilized powders are shipping with blue ice. Solutions are shipping with dry ice.
研究背景
Chimeric antigen receptor (CAR) T cells, a type of cell-based immunotherapy, have shown some promising results in multiple myeloma, a bone marrow cancer.The orphan G protein–coupled receptor, class C group 5 member D (GPRC5D), normally expressed only in the hair follicle, Using quantitative immunofluorescence, we determined that GPRC5D protein is expressed on CD138 MM cells from primary marrow samples with a distribution that was similar to, but independent of, BCMA.

参考文献

SCI 文献

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