CD45 Protein, Mouse, Recombinant (aa 453-1152, His & GST) is expressed in Baculovirus insect cells with His and GST tag. The predicted molecular weight is 108.2 kDa and the accession number is AAA39458.1.
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
100 μg | ¥ 5,750 | 5日内发货 |
生物活性 | 1. Measured by its ability to cleave a substrate, pNitrophenyl phosphate (pNPP). The specific activity is >50,000 pmoles/min/μg. 2. Measured by its ability to bind biotinylated Galectin-1 in a functional ELISA. |
产品描述 | CD45 Protein, Mouse, Recombinant (aa 453-1152, His & GST) is expressed in Baculovirus insect cells with His and GST tag. The predicted molecular weight is 108.2 kDa and the accession number is AAA39458.1. |
种属 | Mouse |
表达系统 | Baculovirus Insect Cells |
标签 | His, GST |
蛋白编号 | AAA39458.1 |
别名 | Ly-5, CD45R, Lyt-4, T200, loc, protein tyrosine phosphatase, receptor type, C, B220, L-CA, Cd45 |
蛋白构建 | The Mouse PTPRC (AAA39458.1)(Arg453-Ser1152) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus. |
蛋白纯度 | > 90 % as determined by SDS-PAGE |
分子量 | 108.2 kDa (predicted) |
内毒素 | < 1.0 EU/μg of the protein as determined by the LAL method. |
缓冲液 | Supplied as sterile 20 mM Tris, 500 mM NaCl, 0.5 mM GSH, 3 mM DTT, pH 7.4, 10% gly. |
复溶方法 | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
存储 |
It is recommended to store the product under sterile conditions at -20℃ to -80℃. Samples are stable for up to 12 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
运输方式 |
Kinases are highly recommended to be shipped at frozen temperature with blue ice or dry ice. |
研究背景 | The cluster of differentiation (CD) system is commonly used as cell markers in Immunophenotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. Protein tyrosine phosphatase, receptor type C (CD45), also known as PTPRC is a member of the protein tyrosine phosphatase (PTP) family which is known for its function to serve as signaling molecules and to regulate a variety of cellular processes such as cell proliferation, differentiation, mitotic cycle and oncogenic transformation. CD45 is found expression specifically in hemotopietic cells. CD45 consists of an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains. It serves as an essential regulator of T-cell and B-cell antigen receptor signaling through either direct interaction with components of the antigen receptor complexes or by activating various Src family kinases required for the antigen receptor signaling and it also can suppress JAK kinases. |
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CD45 Protein, Mouse, Recombinant (aa 453-1152, His & GST) Ly-5 T 200 CD45R Lyt-4 T200 B-220 loc B 220 protein tyrosine phosphatase, receptor type, C B220 L-CA Cd45 T-200 recombinant recombinant-proteins proteins protein