Name: CD19 Protein, Human, Recombinant (His & Avi), Biotinylated
Brand: TargetMol
SKU: TMPY-06755
Rating: 4.5 (1 reviews)

规格

价格

库存

数量

5 μg

¥ 1,350

6-8日内发货

10 μg

¥ 2,270

6-8日内发货

20 μg

¥ 3,820

5日内发货

50 μg

¥ 5,930

5日内发货

100 μg

¥ 9,870

5日内发货

生物活性

产品描述	CD19 Protein, Human, Recombinant (His & Avi), Biotinylated is expressed in HEK293 mammalian cells with His and Avi tag. The predicted molecular weight is 36 kDa and the accession number is P15391-1.
生物活性	Immobilized Biotinylated Human CD19, His-Avi Tag at 2μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Coltuximab, hFc Tag with the EC50 of 6.6ng/ml determined by ELISA (QC Test). Immobilized Biotinylated Human CD19, His Tag at 2μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for FMC63, mFc Tag with the EC50 of 11.4ng/ml determined by ELISA. Anti-CD19 Antibody, hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Human CD19, His Tag with an affinity constant of 0.64 nM as determined in SPR assay (Biacore T200). Loaded Coltuximab on ProA-Biosensor can bind Biotinylated Human CD19, His-Avi Tag with an affinity constant of 4.53 nM as determined in BLI assay (Gator® Prime). Loaded Inebilizumab on ProA-Biosensor can bind Biotinylated Human CD19, His-Avi Tag with an affinity constant of 11.00 nM as determined in BLI assay (Gator® Prime). Loaded Denintuzumab on ProA-Biosensor can bind Biotinylated Human CD19, His-Avi Tag with an affinity constant of4.70 nM as determined in BLI assay (Gator® Prime).
研究背景	CD19 is a 95 kDa transmembrane glycoprotein that plays a central role in B cell activation and humoral immune responses. Functions as coreceptor for the B-cell antigen receptor complex (BCR) on B-lymphocytes. Decreases the threshold for activation of downstream signaling pathways and for triggering B-cell responses to antigens. Activates signaling pathways that lead to the activation of phosphatidylinositol 3-kinase and the mobilization of intracellular Ca2 stores.
种属	Human
表达系统	HEK293 Cells
标签	C-His-Avi
蛋白编号	P15391-1
蛋白构建	Pro20-Lys291
蛋白纯度	> 95% as determined by Bis-Tris PAGE
蛋白性状	Lyophilized powder
缓冲液	Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
复溶方法	Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
别名	CVID3, CD19 molecule, B4
内毒素	< 1.0 EU/μg of the protein as determined by the LAL method.

化学信息

分子量	36 kDa (predicted); 50-65 kDa (reducing conditions)

储存&溶解度

运输方式	In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
存储

CD19 Protein, Human, Recombinant (His & Avi), Biotinylated

CD19 Protein, Human, Recombinant (His & Avi), Biotinylated

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