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Protease Inhibitor Cocktail (EDTA-Free, 100× in DMSO)

Catalog No. C0001

在稳定的环境条件下细胞内蛋白质的生成和分解处于动态平衡,当在体外从细胞和组织中提取蛋白时,粗细胞提取物含有许多能够降解目的蛋白的内源酶,例如磷酸酶和蛋白酶。这些酶从细胞内释放出来或被激活,导致目的蛋白分解,影响分析结果。为了避免这种情况的出现,提高目的蛋白产量的一种方法是加入相关酶的抑制剂。蛋白酶抑制剂有很多种,包括 E-64、NaVO3、Bestatin、Leupetin、PepstatinA、Aprotinin 等均具有抑制蛋白酶的活性的作用。

TargetMol 蛋白酶抑制剂 cocktail 由6个成分组成,包括对半胱氨酸、丝氨酸、天冬氨酸蛋白酶和氨基肽酶具有广泛特异性的 AEBSF、Aprotinin、Bestatin、E-64、Leupeptin 和 Pepstatin A,不含EDTA。已经用哺乳动物细胞和组织提取物对该蛋白酶抑制剂cocktail 进行了优化和测试,TargetMol 蛋白酶抑制剂cocktail 具有高效抑制自动物、植物、细菌、酵母、真菌样品中的丝氨酸蛋白酶,半胱氨酸蛋白酶及金属蛋白酶在内的多种蛋白酶,方便可靠地保护您的蛋白。适用于从多种组织细胞中提取蛋白质,能够更有效的获得目的蛋白质。

TargetMol的所有产品和服务仅用于科学研究,不能被用于人体,我们也不向个人提供产品和服务。
Protease Inhibitor Cocktail (EDTA-Free, 100× in DMSO)
规格 价格/CNY 货期 数量
1 mL ¥ 220 现货
1 mL * 10 ¥ 1,620 现货
1 mL * 50 ¥ 6,500 现货
1 mL * 100 ¥ 9,700 现货
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产品应用

在室温下解冻,开盖前请低速离心一下,以便将黏附于管壁的液体甩至管底。然后在实验前以1:100(v / v)稀释至溶液样品(如细胞裂解液或组织提取液),短暂涡旋震荡加速溶解。应用于:WB、Co-IP、pull-down、IF、IHC、kinase assay等。

产品组成

Ingredient CAS MW Concentration (100x) Target Type
AEBSF 30827-99-7 239.69 104 mM 丝氨酸蛋白酶 不可逆
Aprotinin 9087-70-1 6511.44 80 μM 丝氨酸蛋白酶 可逆
Bestatin 58970-76-6 308.37 5 mM 氨肽酶 B; 亮氨酸氨基肽酶 可逆
E-64 66701-25-5 357.41 1.5 mM 半胱氨酸蛋白酶 不可逆
Leupeptin 55123-66-5 475.6 2 mM 半胱氨酸蛋白酶; 丝氨酸蛋白酶 可逆
Pepstatin A 26305-03-3 685.89 1.5 mM 天冬氨酸蛋白酶 可逆

包装与规格

Attribute Value
Form Liquid
Formulation In 1 mL DMSO
Storage -20 ℃ for one year, Avoid free/thaw
Freeze Ok to freeze
Toxicity Irritant

产品优势

  • 组分清晰,浓度明确;
  • 产品兼容性好,高效抑制多种蛋白酶,全面保护蛋白不被降解。

文献引用

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