MetAP1 is known to work in the presence of various divalent cations, is considered to acts function with a most probable cofactor, iron (Fe 2+ ) [1]. MtMetAP1-IN-1 (compound 12) (12.5 μM; ) inhibits MtMetAP1 under different metals catalytic effect with residual activity RA (%) of 51% (Co 2+ ), 21% (Ni 2+ ), 33% (Fe 2+ ) [1]. MtMetAP1-IN-1 has a preference towards Ni 2+, inhibits MtMetAP1 with an IC 50 value of with 0.7 μM [1].