Anti-Tissue Factor Antibody (3Q104) is an antibody targeting Tissue Factor. Anti-Tissue Factor Antibody (3Q104) can be used in ELISA, WB, IHC.

Rabbit IgG

3Q104

Human, Rat

1. Western Blot
-Positive WB detected in: HL60 whole cell lysate, U251 whole cell lysate, Hela whole cell lysate, NTERA-2 whole cell lysate, K562 whole cell lysate, Jurkat whole cell lysate, Rat brain tissue
-All lanes: F3 antibody at 1:1500
-Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution
-Predicted band size: 34, 28 kDa
-Observed band size: 46 kDa
2. IHC image of TMAH-00413 diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

ELISA, WB, IHC

WB:1:500-1:5000; IHC:1:50-1:200.

Monoclonal

Unconjugated

A synthesized peptide derived from human Tissue Factor

Human

2152

Cardiovascular, Metabolism