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Anti-SULT1A1 Antibody (2N267)
还可以产品编号 TMAY-01965
Anti-SULT1A1 Antibody (2N267) 是一种 Mouse 抗体，靶向 SULT1A1。Anti-SULT1A1 Antibody (2N267) 可用于 WB,ELISA,IP。
Anti-SULT1A1 Antibody (2N267)
Anti-SULT1A1 Antibody (2N267)
还可以产品编号 TMAY-01965
Anti-SULT1A1 Antibody (2N267) 是一种 Mouse 抗体，靶向 SULT1A1。Anti-SULT1A1 Antibody (2N267) 可用于 WB,ELISA,IP。
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偶联与修饰
抗原信息
| 产品描述 | Anti-SULT1A1 Antibody (2N267) is a Mouse antibody targeting SULT1A1. Anti-SULT1A1 Antibody (2N267) can be used in WB,ELISA,IP. |
| Ig Type | Monoclonal Mouse IgG1 |
| 克隆号 | 2N267 |
| 交叉反应 | Human |
| 验证活性 | 1. Anti-SULT1A1 mouse monoclonal antibody at 1:500 dilution. -Lane A: HepG2 Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution. -Developed using the Odyssey technique. -Performed under reducing conditions. -Predicted band size:34 kDa. -Observed band size:34 kDa. 2. SULT1A1 was immunoprecipitated using: -Lane A:0.5 mg HepG2 Whole Cell Lysate. -2 µL anti-SULT1A1 mouse monoclonal antibody and 15 μl of 50 % Protein G agarose. -Primary antibody: -Anti-SULT1A1 mouse monoclonal antibody, at 1:100 dilution. -Secondary antibody: -Dylight 800-labeled antibody to Mouse IgG (H+L), at 1:7500 dilution. -Developed using the odyssey technique. -Performed under reducing conditions. -Predicted band size: 34 kDa. -Observed band size: 34 kDa. 3. Anti-SULTA1 mouse monoclonal antibody at 1:500 dilution. -Lane A: HepG2 Whole Cell Lysate. -Lane B: SULTA1 konckout HepG2 Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Mouse IgG (H+L)/HRP at 1/10000 dilution. -Developed using the ECL technique. -Performed under reducing conditions. -Predicted band size:34 kDa. -Observed band size:34 kDa |
| 应用 | WB,ELISA,IP |
| 推荐剂量 | WB: 1:500-1:2000; ELISA: 1:1000-1:2000; IP: 1-4 μL/mg of lysate |
| 抗体种类 | Monoclonal |
| 宿主来源 | Mouse |
| 构建方式 | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human SULT1A1 (rh SULT1A1; TMPY-02062; P50225-1; Glu 2-Leu 295). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
| 纯化方式 | Protein A |
| 性状 | Liquid |
| 缓冲液 | 0.2 μm filtered solution in PBS |
| 研究背景 | Sulfate conjugation catalyzed by cytosolic sulfotransferase (SULT) enzymes. The SULTs are Phase II drug-metabolizing enzymes that catalyze the addition of a sulfuryl moiety to both endogenous compounds, including steroids and neurotransmitters, and certain xenobiotics, including N-hydroxy-2-acetylaminoflourine and phenolic compounds, like alpha-naphthol. SULTs may be involved in the individual genetic disposition, species differences, and organotropisms for toxicological effects of chemicals. Particularly SULT1A1 (Sulfotransferase family, cytosolic, 1A, phenol-preferring, member 1), a member of the sulfotransferase 1 subfamily, which is a major pathway for drug metabolism in humans. Humans have at least 10 functional SULT genes. There has been an explosion in information on sulfotransferase polymorphisms and their functional consequences. An Arg213His polymorphism in SULT1A1 has a strong influence on the level of enzyme protein and activity in platelets, which have been widely used for phenotyping. Statistically significant associations were observed between the SULT1A1 genotype (Arg213His) and age, obesity and certain neoplasias (mammary, pulmonary, esophageal and urothelial cancer). Furthermore, the polymorphism of the SULT1A1 may be closely associated with breast cancer. |
| 偶联 | Unconjugated |
| 免疫原 | Recombinant Human SULT1A1 protein (TMPY-02062) |
| 抗原种属 | Human |
存储&运输
| 储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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