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Anti-PTP1B Antibody (5M68)
还可以产品编号 TMAY-00587
Anti-PTP1B Antibody (5M68) 是一种 Rabbit 抗体，靶向 PTP1B。Anti-PTP1B Antibody (5M68) 可用于 WB,ELISA,IP。
Anti-PTP1B Antibody (5M68)
Anti-PTP1B Antibody (5M68)
还可以产品编号 TMAY-00587
Anti-PTP1B Antibody (5M68) 是一种 Rabbit 抗体，靶向 PTP1B。Anti-PTP1B Antibody (5M68) 可用于 WB,ELISA,IP。
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产品介绍
生物活性
偶联与修饰
抗原信息
| 产品描述 | Anti-PTP1B Antibody (5M68) is a Rabbit antibody targeting PTP1B. Anti-PTP1B Antibody (5M68) can be used in WB,ELISA,IP. |
| Ig Type | Monoclonal Rabbit IgG |
| 克隆号 | 5M68 |
| 交叉反应 | Human |
| 验证活性 | 1. Anti-PTPN1 rabbit monoclonal antibody at 1:500 dilution. -Lane A: Hela whole cell Lysate. -Lane B: MCF7 whole cell Lysate. -Lane C: A549 whole cell Lysate. -Lane D: Jurkat whole cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. -Developed using the Odyssey technique. -Performed under reducing conditions. -Predicted band size:50 kDa. -Observed band size:53 kDa. 2. PTPN1 was immunoprecipitated using: -Lane A:0.5 mg A549 Whole Cell Lysate. -2 µL anti-PTPN1 rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose. -Primary antibody: -Anti-PTPN1 rabbit monoclonal antibody, at 1:330 dilution. -Secondary antibody: -Clean-Blotô IP Detection Reagent (HRP) at 1:1000 dilution. -Developed using the DAB staining technique. -Performed under reducing conditions. -Predicted band size: 50 kDa. -Observed band size: 50 kDa. 3. Anti-PTPN1 rabbit monoclonal antibody at 1:500 dilution. -Lane A: PTPN1 konckout Hela Cell Lysate. -Lane B: Hela Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Rabbit IgG H&L /HRP at 1/10000 dilution. -Developed using the ECL technique. -Performed under reducing conditions. -Predicted band size:50 kDa. -Observed band size:53 kDa(Validation Experiment) |
| 应用 | WB,ELISA,IP |
| 推荐剂量 | WB: 1:500-1:2000; ELISA: 1:5000-1:10000; IP: 1-4 μL/mg of lysate |
| 抗体种类 | Monoclonal |
| 宿主来源 | Rabbit |
| 构建方式 | This antibody was obtained from a rabbit immunized with purified, recombinant Human PTP1B / PTPN1 (rh PTP1B / PTPN1; TMPY-04125; NP_002818.1; Glu 2-Asn 321). |
| 纯化方式 | Protein A |
| 性状 | Liquid |
| 缓冲液 | 0.2 μm filtered solution in PBS |
| 研究背景 | PTP1B, also known as PTPN1, belongs to the protein-tyrosine phosphatase (PTP) family. PTPs catalyze the hydrolysis of the phosphate monoesters specifically on tyrosine residues. Members of the PTP family share a highly conserved catalytic motif, which is essential for the catalytic activity. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. PTP1B contains 1 tyrosine-protein phosphatase domain and is expressed in many tissues. PTP1B is localized to the cytoplasmic face of the endoplasmic reticulum. PTP1B was also reported to dephosphorylate epidermal growth factor receptor kinase, as well as JAK2 and TYK2 kinases, which implicated the role of PTP1B in cell growth control, and cell response to IFN stimulation. |
| 偶联 | Unconjugated |
| 免疫原 | Recombinant Human PTP1B / PTPN1 protein (TMPY-04125) |
| 抗原种属 | Human |
| 研究领域 | Phosphatases and Regulators |
存储&运输
| 储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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