Anti-Phospho-EIF2AK2 (Thr446) Antibody (4K902) is an antibody targeting Phospho-EIF2AK2 (Thr446). Anti-Phospho-EIF2AK2 (Thr446) Antibody (4K902) can be used in ELISA, WB, IHC, IF.

Rabbit IgG

4K902

Human

1. Western Blot
-Positive WB detected in Hela whole cell lysate(treated with Calyculin A or not)
-All lanes Phospho-EIF2AK2 antibody at 1.25μg/ml
-Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution
-Predicted band size: 62 KDa
-Observed band size: 62 KDa
2. IHC image of TMAH-00892 diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
3. Immunofluorescence staining of Hela cells(treated with 50mM Calyculin A for 30min) with TMAH-00892 at 1:100,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).

WB:1:500-1:5000; IHC:1:50-1:200; IF:1:20-1:200.

Monoclonal

Unconjugated

A synthetic peptide: Human Phospho-EIF2AK2 (T446)

Human

EIF2AK2

Signal Transduction