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Anti-IKBKB Antibody (3T443)
还可以产品编号 TMAH-00604
别名 IKBKB
Anti-IKBKB Antibody (3T443) 是一种抗体，靶向 IKBKB。Anti-IKBKB Antibody (3T443) 可用于 ELISA, WB, IHC, IF。
Anti-IKBKB Antibody (3T443)
Anti-IKBKB Antibody (3T443)
还可以产品编号 TMAH-00604 别名 IKBKB
Anti-IKBKB Antibody (3T443) 是一种抗体，靶向 IKBKB。Anti-IKBKB Antibody (3T443) 可用于 ELISA, WB, IHC, IF。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,305 | 5日内发货 | |
| 100 μL | ¥ 2,185 | 5日内发货 |
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产品介绍
生物活性
偶联与修饰
抗原信息
| 产品描述 | Anti-IKBKB Antibody (3T443) is an antibody targeting IKBKB. Anti-IKBKB Antibody (3T443) can be used in ELISA, WB, IHC, IF. |
| 别名 | IKBKB |
| Ig Type | Rabbit IgG |
| 克隆号 | 3T443 |
| 交叉反应 | Human |
| 验证活性 | 1. Western Blot -Positive WB detected in: K562 whole cell lysate, MCF-7 whole cell lysate, U251 whole cell lysate -All lanes: IKK beta antibody at 1:2000 -Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution -Predicted band size: 87 kDa -Observed band size: 87 kDa 2. IHC image of TMAH-00604 diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB. 3. Immunofluorescence staining of Hela Cells with TMAH-00604 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L). |
| 应用 | ELISA, WB, IHC, IF |
| 推荐剂量 | WB:1:500-1:5000; IHC:1:50-1:200; IF:1:20-1:200. |
| 抗体种类 | Monoclonal |
| 亚细胞定位 | Cytoplasm. Nucleus. Membrane raft. |
| 构建方式 | Recombinant Antibody |
| 纯化方式 | Affinity-chromatography |
| 性状 | Liquid |
| 缓冲液 | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| 研究背景 | Serine kinase that plays an essential role in the NF-kappa-B signaling pathway which is activated by multiple stimuli such as inflammatory cytokines, bacterial or viral products, DNA damages or other cellular stresses. Acts as part of the canonical IKK complex in the conventional pathway of NF-kappa-B activation. Phosphorylates inhibitors of NF-kappa-B on 2 critical serine residues. These modifications allow polyubiquitination of the inhibitors and subsequent degradation by the proteasome. In turn, free NF-kappa-B is translocated into the nucleus and activates the transcription of hundreds of genes involved in immune response, growth control, or protection against apoptosis. In addition to the NF-kappa-B inhibitors, phosphorylates several other components of the signaling pathway including NEMO/IKBKG, NF-kappa-B subunits RELA and NFKB1, as well as IKK-related kinases TBK1 and IKBKE. IKK-related kinase phosphorylations may prevent the overproduction of inflammatory mediators since they exert a negative regulation on canonical IKKs. Phosphorylates FOXO3, mediating the TNF-dependent inactivation of this pro-apoptotic transcription factor. Also phosphorylates other substrates including NCOA3, BCL10 and IRS1. Within the nucleus, acts as an adapter protein for NFKBIA degradation in UV-induced NF-kappa-B activation. Phosphorylates RIPK1 at 'Ser-25' which represses its kinase activity and consequently prevents TNF-mediated RIPK1-dependent cell death. Phosphorylates the C-terminus of IRF5, stimulating IRF5 homodimerization and translocation into the nucleus. |
| 偶联 | Unconjugated |
| 免疫原 | A synthetic peptide: Human IKK beta |
| 抗原种属 | Human |
| 基因ID | 3551 |
| Uniprot ID | |
| 研究领域 | Epigenetics and Nuclear Signaling, Cardiovascular, Immunology, Signal transduction |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
关键词
Serine/threonine protein kinase IKBKBNuclear factor NF-kappa-B inhibitor kinase β (NFKBIKB)Nuclear factor NF-kappa-B inhibitor kinase beta (NFKBIKB)Nuclear factor NF-kappa-B inhibitor kinase b (NFKBIKB)NFKBIKBInhibitor of nuclear factor kappa-B kinase subunit βInhibitor of nuclear factor kappa-B kinase subunit betaInhibitor of nuclear factor kappa-B kinase subunit bIKK-βIKKβIKK-betaIKK-BIKKBIKK-2IKK2I-kappa-B-kinase βI-kappa-B-kinase betaI-kappa-B-kinase bI-kappa-B kinase 2 (IKK-2;IKK2)
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