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Anti-EIF4A1 Antibody (5Z864)
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产品编号 TMAH-00382
Anti-EIF4A1 Antibody (5Z864) 是一种抗体，靶向 EIF4A1。Anti-EIF4A1 Antibody (5Z864) 可用于 ELISA, WB, IF。
Anti-EIF4A1 Antibody (5Z864)
Anti-EIF4A1 Antibody (5Z864)
还可以产品编号 TMAH-00382
Anti-EIF4A1 Antibody (5Z864) 是一种抗体，靶向 EIF4A1。Anti-EIF4A1 Antibody (5Z864) 可用于 ELISA, WB, IF。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,315 | 5日内发货 | |
| 100 μL | ¥ 2,185 | 5日内发货 |
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产品介绍
生物活性
偶联与修饰
抗原信息
| 产品描述 | Anti-EIF4A1 Antibody (5Z864) is an antibody targeting EIF4A1. Anti-EIF4A1 Antibody (5Z864) can be used in ELISA, WB, IF. |
| Ig Type | Rabbit IgG |
| 克隆号 | 5Z864 |
| 交叉反应 | Human |
| 验证活性 | 1. Western Blot -Positive WB detected in: K562 whole cell lysate, 293 whole cell lysate, RAW264.7 whole cell lysate -All lanes: eIF4A1 Antibody at 1:1000 -Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution -Predicted band size: 40, 47 kDa -Observed band size: 50 kDa 2. Immunofluorescence staining of Hela Cells with TMAH-00382 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L). |
| 应用 | |
| 推荐剂量 | WB:1:500-1:5000; IF:1:20-1:200. |
| 抗体种类 | Monoclonal |
| 构建方式 | Recombinant Antibody |
| 纯化方式 | Affinity-chromatography |
| 性状 | Liquid |
| 缓冲液 | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| 研究背景 | ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon. |
| 偶联 | Unconjugated |
| 免疫原 | A synthetic peptide: Human eIF4A1 |
| 抗原种属 | Human |
| 基因ID | |
| Uniprot ID | |
| 研究领域 | Epigenetics and Nuclear Signaling |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
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