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Anti-EIF2AK2 Antibody (3D60)
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产品编号 TMAC-03357
别名 eIF2A protein kinase2
Anti-EIF2AK2 Antibody (3D60) 是一种 Rabbit 抗体，靶向 EIF2AK2。Anti-EIF2AK2 Antibody (3D60) 可用于 WB,ICC/IF,IHC,IP。
Anti-EIF2AK2 Antibody (3D60)
Anti-EIF2AK2 Antibody (3D60)
还可以产品编号 TMAC-03357 别名 eIF2A protein kinase2
Anti-EIF2AK2 Antibody (3D60) 是一种 Rabbit 抗体，靶向 EIF2AK2。Anti-EIF2AK2 Antibody (3D60) 可用于 WB,ICC/IF,IHC,IP。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,480 | 5日内发货 | |
| 100 μL | ¥ 2,480 | 5日内发货 |
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产品介绍
生物活性
偶联与修饰
抗原信息
化学信息
| 产品描述 | Anti-EIF2AK2 Antibody (3D60) is a Rabbit antibody targeting EIF2AK2. Anti-EIF2AK2 Antibody (3D60) can be used in WB,ICC/IF,IHC,IP. |
| 别名 | eIF2A protein kinase2 |
| Ig Type | IgG |
| 交叉反应 | Human |
| 验证活性 | 1. Western blot analysis of PKR on different lysates using anti-PKR antibody at 1/1,000 dilution. Positive control: Lane 1: MCF-7, Lane 2: Hela. 2. Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-PKR antibody. Counter stained with hematoxylin. 3. ICC staining PKR in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
| 应用 | |
| 推荐剂量 | WB: 1:1000-5000; IHC: 1:50-100; ICC/IF: 1:50-100 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Rabbit |
| 构建方式 | Recombinant Antibody |
| 纯化方式 | ProA affinity purified |
| 性状 | Liquid |
| 缓冲液 | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| 研究背景 | An interferon-inducible, RNA-dependent protein serine/threonine kinase (PKR) has been described. PKR in earlier literature is variously known as DAI, dsJ, PI kinase, p65, p67 or TIK for the mouse kinase; and p68 or p69 for the human kinase. The PKR kinase substrate is the a subunit of protein synthesis initiation factor eIF-2. Phosphorylation of eIF-2a on serine-51 results in inhibition of translation. Molecular cDNA clones have been isolated from both human and mouse cells. The serine/threonine kinase catalytic domains map to the carboxy terminal half of the protein while the RNA-binding domains are located in the amino terminal region. Three kinds of regulation of PKR enzymatic activity have been described. These include transcriptional regulation in response to interferon, an autoregulatory mechanism controlling PKR expression at the level of translation and post-translational regulation by RNA mediated autophosphorylation. |
| 偶联 | Unconjugated |
| 免疫原 | Recombinant Protein |
| Uniprot ID |
| 分子量 | Theoretical: 68 kDa. |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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关键词
Tyrosine-protein kinase EIF2AK2Protein kinase RNA-activated (PKRProtein kinase R)PRKRPKRp68 kinaseP1/eIF-2A protein kinaseInterferon-inducible RNA-dependent protein kinaseInterferon-induced, double-stranded RNA-activated protein kinaseEukaryotic translation initiation factor 2-α kinase 2 (eIF-2A protein kinase 2)Eukaryotic translation initiation factor 2-alpha kinase 2 (eIF-2A protein kinase 2)Eukaryotic translation initiation factor 2-a kinase 2 (eIF-2A protein kinase 2)EIF2AK2EIF2AK 2eIF-2A protein kinase 2double-stranded RNA-activated protein kinase
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