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Anti-DOT1L Antibody (6U296)
还可以产品编号 TMAH-00361
别名 KMT4, DOT1-like histone H3K79 methyltransferase, DOT1
Anti-DOT1L Antibody (6U296) 是一种抗体，靶向 DOT1L。Anti-DOT1L Antibody (6U296) 可用于 ELISA, WB。
Anti-DOT1L Antibody (6U296)
Anti-DOT1L Antibody (6U296)
还可以产品编号 TMAH-00361 别名 KMT4, DOT1-like histone H3K79 methyltransferase, DOT1
Anti-DOT1L Antibody (6U296) 是一种抗体，靶向 DOT1L。Anti-DOT1L Antibody (6U296) 可用于 ELISA, WB。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,315 | 5日内发货 | |
| 100 μL | ¥ 2,180 | 5日内发货 |
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产品介绍
生物活性
偶联与修饰
抗原信息
| 产品描述 | Anti-DOT1L Antibody (6U296) is an antibody targeting DOT1L. Anti-DOT1L Antibody (6U296) can be used in ELISA, WB. |
| 别名 | KMT4, DOT1-like histone H3K79 methyltransferase, DOT1 |
| Ig Type | Rabbit IgG |
| 克隆号 | 6U296 |
| 交叉反应 | Human |
| 验证活性 | 1. Western Blot -Positive WB detected in: A549 whole cell lysate, Hela whole cell lysate, 293T whole cell lysate -All lanes: DOT1L antibody at 1:1000 -Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution -Predicted band size: 165 kDa -Observed band size: 165 kDa 2. Immunofluorescence staining of Hela cell with TMAH-00361 at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
| 应用 | ELISA, WB |
| 推荐剂量 | WB:1:500-1:5000. |
| 抗体种类 | Monoclonal |
| 亚细胞定位 | Nucleus. |
| 构建方式 | Recombinant Antibody |
| 纯化方式 | Affinity-chromatography |
| 性状 | Liquid |
| 缓冲液 | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| 研究背景 | Histone methyltransferase. Methylates 'Lys-79' of histone H3. Nucleosomes are preferred as substrate compared to free histones. Binds to DNA. |
| 偶联 | Unconjugated |
| 免疫原 | A synthetic peptide: Human KMT4 / Dot1L |
| 抗原种属 | Human |
| 基因ID | 84444 |
| Uniprot ID | |
| 研究领域 | Epigenetics and Nuclear Signaling |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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