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顶部生物活性储存和溶解信息计算器

Anti-CD19 Antibody (7Q387)

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产品编号 TMAH-00177

别名 CVID3, CD19 molecule, B4

Anti-CD19 Antibody (7Q387) 是一种抗体，靶向 CD19。Anti-CD19 Antibody (7Q387) 可用于 ELISA,IHC,IP,WB。

Anti-CD19 Antibody (7Q387)

Anti-CD19 Antibody (7Q387)

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产品编号 TMAH-00177 别名 CVID3, CD19 molecule, B4

Anti-CD19 Antibody (7Q387) 是一种抗体，靶向 CD19。Anti-CD19 Antibody (7Q387) 可用于 ELISA,IHC,IP,WB。

规格价格库存数量
50 μL
¥ 1,315
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100 μL
¥ 2,190
5日内发货
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产品介绍

生物活性
产品描述
Anti-CD19 Antibody (7Q387) is an antibody targeting CD19. Anti-CD19 Antibody (7Q387) can be used in ELISA,IHC,IP,WB.
别名CVID3, CD19 molecule, B4
Ig Type
Rabbit IgG
克隆号
7Q387
交叉反应
Human
验证活性
1. Western Blot
-Positive WB detected in: Daudi whole cell lysate, Raji whole cell lysate
-All lanes: CD19 antibody at 1:2000
-Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution
-Predicted band size: 62 kDa
-Observed band size: 95 kDa
2. IHC image of TMAH-00177 diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
3. Immunoprecipitating CD19 in Raji whole cell lysate
-Lane 1: Rabbit control IgG instead of TMAH-00177 in Raji whole cell lysate.
For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
-Lane 2: TMAH-00177(2µg)+ Raji whole cell lysate(500µg)
-Lane 3: Raji whole cell lysate (10µg)
应用ELISAIHCIPWB
推荐剂量
WB:1:500-1:5000; IHC:1:50-1:200; IP:1:200-1:1000.
抗体种类
Monoclonal
亚细胞定位Cell membrane; Single-pass type I membrane protein. Membrane raft; Single-pass type I membrane protein.
组织特异性Detected on marginal zone and germinal center B cells in lymph nodes. Detected on blood B cells (at protein level).
构建方式Recombinant Antibody
纯化方式Affinity-chromatography
性状Liquid
缓冲液Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
研究背景Functions as coreceptor for the B-cell antigen receptor complex (BCR) on B-lymphocytes. Decreases the threshold for activation of downstream signaling pathways and for triggering B-cell responses to antigens. Activates signaling pathways that lead to the activation of phosphatidylinositol 3-kinase and the mobilization of intracellular Ca(2+) stores. Is not required for early steps during B cell differentiation in the blood marrow. Required for normal differentiation of B-1 cells. Required for normal B cell differentiation and proliferation in response to antigen challenges. Required for normal levels of serum immunoglobulins, and for production of high-affinity antibodies in response to antigen challenge.
偶联与修饰
偶联
Unconjugated
抗原信息
免疫原
A synthetic peptide: Human CD19
抗原种属
Human
基因名称
CD19
基因ID
930
Uniprot ID
P15391
研究领域
Immunology, Stem cells
存储&运输
储存方式Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

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关键词

CVID-3CVID 3B-4B 4

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