suc-yvad-amc

Suc-YVAD-AMC is a fluorogenic substrate for caspase-1. Upon enzymatic cleavage by caspase-1, 7-amino-4-methylcoumarin (AMC) is released and its fluorescence can be used to quantify caspase-1 activity. AMC displays excitation/emission maxima of 340-360/440-460 nm, respectively.

Suc-YVAD-AMC 用于检测蛋白酶体中的半胱氨酸蛋白酶。酶切Suc-YVAD-AMC后，AMC荧光分子被释放，用以评估蛋白酶体的酶活性。