Vaccinia virus (strain Copenhagen) L1 重组蛋白 (His & Myc)

Vaccinia virus (strain Copenhagen) L1 Protein (His & Myc)

产品编号 TMPH-03673 Vaccinia virus (strain Copenhagen) L1 Protein (His & Myc)

Envelope protein which probably plays a role in virus entry into the host cell. Is probably involved in the virus attachment to the host cell surface and associates with the entry/fusion complex (EFC). Needed for fusion and penetration of the virus core into host cell. Vaccinia virus (strain Copenhagen) L1 Protein (His & Myc) is expressed in Baculovirus insect cells with N-10xHis and C-Myc tag. The predicted molecular weight is 23.2 kDa and the accession number is P20540.

Vaccinia virus (strain Copenhagen) L1 Protein (His & Myc)

产品编号 TMPH-03673 Vaccinia virus (strain Copenhagen) L1 Protein (His & Myc)

规格	价格	库存
20 μg	¥ 3,120	20日内发货
100 μg	¥ 9,630	20日内发货
1 mg	¥ 19,200	20日内发货

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常见问题解答

问细胞实验中需要加多少抑制剂？

答通常建议您参考同模型实验发表的文献报道；除参考文献报道以外，还需要通过预实验做“剂量-效应曲线”确定最佳作用浓度（浓度梯度）；通过“时间-效应曲线”确定最佳孵育时间（处理时间梯度）。另外，抑制剂的使用量受多种因素影响，包括抑制对象的可接触性、细胞通透性、孵育时间、细胞种类等等。我们建议通过检索文献确定使用抑制剂的起始浓度。如果有报道的 Ki 值或 IC50 值，可以采用其 5-10 倍的量开始尝试以达到抑制酶活性的最佳效果。如果抑制剂的 Ki 值或 IC50 值未知，则需要在更广泛的范围尝试抑制剂的使用浓度，并采用 Michaelis-Menten 动力学计算 Ki 值。一般设立溶解抑制剂时所采用的溶剂作为对照，以排除溶剂的非特异性影响。

问溶解度写的“mg/mL”和“mM”是什么意思？

答mg/mL 与 mM，是两种不同单位的表示方式，所指代的最大溶解度是一样的，可以通过官网网页下方的摩尔浓度计算器进行换算

问抑制剂加 DMSO 配成母液之后，能否放在常温的环境保存1周？

答不建议这么做，母液配置好分装保存，建议存于 -20℃ 或 -80℃，尽量避免反复冻融。如果您订购的规格很大，可以考虑分装粉末保存，固体粉末是比较稳定的，-20℃ 可以保存三年。

问超声应使用什么仪器？

答建议使用“超声清洗仪”，不推荐用“超声破碎仪”。

问抑制剂能否用于动物实验？

答可以的，我们的抑制剂都可以用于动物/体内实验。但有些化合物还没有用于动物实验的文献，这种情况下，建议您先做预实验，确保研究的可行性。

产品信息

生物活性	Activity has not been tested. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first.
产品描述	Envelope protein which probably plays a role in virus entry into the host cell. Is probably involved in the virus attachment to the host cell surface and associates with the entry/fusion complex (EFC). Needed for fusion and penetration of the virus core into host cell. Vaccinia virus (strain Copenhagen) L1 Protein (His & Myc) is expressed in Baculovirus insect cells with N-10xHis and C-Myc tag. The predicted molecular weight is 23.2 kDa and the accession number is P20540.
种属	VACV
表达系统	Baculovirus Insect Cells
标签	N-10xHis, C-Myc
蛋白编号	P20540
别名	Virion membrane protein M25,Protein L1,OPG099,Entry-fusion complex associated protein OPG095,EFC-associated protein OPG095
氨基酸序列	GAAASIQTTVNTLSERISSKLEQEANASAQTKCDIEIGNFYIRQNHGCNLTVKNMCSADADAQLDAVLSAATETYSGLTPEQKAYVPAMFTAALNIQTSVNTVVRDFENYVKQTCNSSAVVDNKLKIQNVIIDECYGAPGSPTNLEFINTGSSKGNCAIKALMQLTTKATTQIAPRQVAGTG
蛋白构建	2-183 aa
蛋白纯度	> 85% as determined by SDS-PAGE.
分子量	23.2 kDa (predicted)
内毒素	< 1.0 EU/μg of the protein as determined by the LAL method.
缓冲液	If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
复溶方法	Reconstitute the lyophilized protein in sterile deionized water. The product concentration should not be less than 100 μg/mL. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
存储	Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式	In general, Lyophilized powders are shipping with blue ice. Solutions are shipping with dry ice.
研究背景	Envelope protein which probably plays a role in virus entry into the host cell. Is probably involved in the virus attachment to the host cell surface and associates with the entry/fusion complex (EFC). Needed for fusion and penetration of the virus core into host cell.

Vaccinia virus (strain Copenhagen) L1 Protein (His & Myc)

Vaccinia virus (strain Copenhagen) L1 Protein (His & Myc)

产品信息

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