TMEM27 重组蛋白, Human (His)

TMEM27 Protein, Human, Recombinant (His)

产品编号 TMPY-03365

TMEM27 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 15.8 kDa and the accession number is A0A024R127.

规格	价格	库存	数量
100 μg	¥ 3,820	5日内发货

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常见问题解答

问产品的溶解度是多少？

答建议您先查询TargetMol官网、陶术官网，看该抑制剂的产品介绍，找到溶解度信息。如果没有相应信息，建议联系技术对该化合物的溶解度进行测定。

问期货产品没有纯度啊？这个纯度在哪里看

答期货产品的话，是没有纯度信息，也没有质检信息的。有了现货之后我们会进行质检，测出试剂的纯度和结构等相关信息，并且展示在官网的活性描述上方。

问如何设置母液浓度？

答母液浓度需低于官网给出的溶解度，在这个范围内，根据工作液浓度设置母液浓度，细胞实验中，建议母液浓度设定在工作液浓度的 1000 倍以上。

问是否能直接用缓冲液对抑制剂 DMSO 母液做梯度稀释？

答大部分情况下，都是可以溶解的。但有时，有机试剂直接加入水相介质时会析出。建议将抑制剂先以DMSO做梯度稀释，再将经过稀释的抑制剂加入缓冲液或细胞培养基。有些抑制剂甚至只有在其工作浓度下才能溶于水相。比如细胞实验中希望终浓度为 1 μM 的话，可以把 10 mM 的 DMSO 母液用 DMSO 稀释到 1 mM，再吸 2 μL 加入到 2 mL 的生理盐水/PBS/细胞培液，终浓度即为 1 μM。为避免药物析出，稀释前，可将母液和培养基 37℃ 预热，避免温度低造成严重析出。若稀释过程中出现化合物析出的情况，建议您采用超声加热的方法使其复溶。

问如何选择合适的溶剂？

答常用的溶剂包括DMSO、水、乙醇等。建议您根据抑制剂给出的溶解度参考，选择合适的溶剂。如果该抑制剂可以溶于DMSO，建议使用没有吸潮新开封的 DMSO，如果有潮气污染，很容易带来抑制剂降解或难溶的问题。如果该抑制剂可以溶于水，建议您根据实验类型选择无菌水、生理盐水、无菌 PBS 、培养基等作为溶剂。

产品信息

生物活性	Activity testing is in progress. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first.
产品描述	TMEM27 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 15.8 kDa and the accession number is A0A024R127.
种属	Human
表达系统	HEK293 Cells
标签	C-His
蛋白编号	Q9HBJ8
别名	transmembrane protein 27,NX-17,NX17
蛋白构建	A DNA sequence encoding the human TMEM27 (NP_065716.1) (Met1-Pro141) was expressed with a polyhistidine tag at the C-terminus. Predicted N terminal: Glu 15
蛋白纯度	> 85 % as determined by SDS-PAGE
分子量	15.8 kDa (predicted); 27 and 33 kDa (reducing conditions)
内毒素	< 1.0 EU/μg of the protein as determined by the LAL method.
缓冲液	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
复溶方法	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
存储	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式	In general, Lyophilized powders are shipping with blue ice.
研究背景	TMEM27 is a membrane protein. It has been proposed as a beta cell mass biomarker since it is cleaved and shed by pancreatic beta cells. Overexpression of TMEM27 leads to increased thymidine incorporation, whereas silencing of Tmem27 using RNAi results in a reduction of cell replication. Furthermore, transgenic mice with increased expression of Tmem27 in pancreatic beta cells exhibit increased beta cell mass. TMEM27 is also important for trafficking amino acid transporters to the apical brush border of proximal tubules.

TMEM27 Protein, Human, Recombinant (His)

TMEM27 Protein, Human, Recombinant (His)

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