TEM1/CD248 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with C-His tag. The predicted molecular weight is 72.7 kDa and the accession number is Q9HCU0-1.
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 100 μg | ¥ 3,100 | 5日内发货 | |
| 500 μg | ¥ 12,400 | 5日内发货 | |
| 1 mg | ¥ 20,700 | 5日内发货 |
| 生物活性 | Immobilized Human TEM1, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-TEM1 Antibody, hFc Tag with the EC50 of 4.8ng/ml determined by ELISA. |
| 产品描述 | TEM1/CD248 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with C-His tag. The predicted molecular weight is 72.7 kDa and the accession number is Q9HCU0-1. |
| 种属 | Human |
| 表达系统 | HEK293 Cells |
| 标签 | C-His |
| 蛋白编号 | Q9HCU0-1 |
| 别名 | TEM1,MGC119479,Endosialin,CD248 molecule,CD248,CD164L1,CD164 sialomucin-like 1 |
| 蛋白构建 | Gln18-Leu687 |
| 蛋白纯度 | > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
| 分子量 | 72.7 kDa (predicted). Due to glycosylation, the protein migrates to 115-140 kDa based on Tris-Bis PAGE result. |
| 内毒素 | < 1 EU/μg by the LAL method. |
| 缓冲液 | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization. |
| 复溶方法 | Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing. |
| 存储 | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
| 运输方式 | In general, Lyophilized powders are shipping with blue ice. |
| 研究背景 | Colorectal cancer (CRC) is one of the most common cancers worldwide usually diagnosed in the advanced stage. The serum concentration of tumor endothelial marker 1 (TEM1) was measured and correlated with clinicopathological features to evaluate whether TEM1 might serve as a biomarker for early CRC diagnosis, progression, and prognosis. TEM1 can act as a potential diagnostic, progression, and prognostic serum biomarker for patients with CRC; TEM1 might be a good supplement for commonly used markers CEA and Ca 19-9. |
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