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TEM1/CD248 Protein, Human, Recombinant (His)

产品编号 TMPK-01191

TEM1/CD248 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with C-His tag. The predicted molecular weight is 72.7 kDa and the accession number is Q9HCU0-1.

TEM1/CD248 Protein, Human, Recombinant (His)

TEM1/CD248 Protein, Human, Recombinant (His)

产品编号 TMPK-01191
TEM1/CD248 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with C-His tag. The predicted molecular weight is 72.7 kDa and the accession number is Q9HCU0-1.
规格价格库存数量
100 μg
¥ 3,100
5日内发货
500 μg
¥ 12,400
5日内发货
1 mg
¥ 20,700
5日内发货
大包装 & 定制
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实验操作小课堂
常见问题解答
抑制剂加 DMSO 配成母液之后,能否放在常温的环境保存1周?
不建议这么做,母液配置好分装保存,建议存于 -20℃ 或 -80℃,尽量避免反复冻融。如果您订购的规格很大,可以考虑分装粉末保存,固体粉末是比较稳定的,-20℃ 可以保存三年。
准备进行动物实验,收到大包装粉末后,如何分装溶解?
根据配制出来的溶液是不是澄清的确定一次性能配多少:如果配制出来是澄清溶液,可以一次性多配制一些,储存于4度冰箱,大概一周配一次,放久了可能会失效;如果配制出来是混悬液,建议每次使用的时候都现配现用。
收到化合物后,是否需要称量后配制储备液?
对于小包装,如 1 mg、5 mg、10 mg 等,不建议二次称量。这样会导致化合物损耗,且无法估算。TargetMol 所有产品原装进口,精准称量,您收到后可直接添加相应量溶剂。 对于大包装,如果您需要分批次实验,可每次称量后配制储备液,建议您 10 mg 起称,称量的误差会小一些;当然条件允许,也可以先配制成高浓度母液,分装备用。
产品的溶解度是多少?
建议您先查询TargetMol官网、陶术官网,看该抑制剂的产品介绍,找到溶解度信息。如果没有相应信息,建议联系技术对该化合物的溶解度进行测定。
Tween-20 可以用 Tween-80 替代吗?
不建议使用 Tween-20。相较于 Tween-20,Tween-80 的耐受性更好,Tween-80已被用作评估实验药物和毒物的行为影响的工具,且没有明显的副作用。 参考文献:Castro CA, Hogan JB, Benson KA, Shehata CW, Landauer MR. Behavioral effects of vehicles: DMSO, ethanol, Tween-20, Tween-80, and emulphor-620. Pharmacol Biochem Behav. 1995 Apr;50(4):521-6.
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产品信息

生物活性
Immobilized Human TEM1, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-TEM1 Antibody, hFc Tag with the EC50 of 4.8ng/ml determined by ELISA.
产品描述
TEM1/CD248 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with C-His tag. The predicted molecular weight is 72.7 kDa and the accession number is Q9HCU0-1.
种属
Human
表达系统
HEK293 Cells
标签C-His
蛋白编号Q9HCU0-1
别名
TEM1,MGC119479,Endosialin,CD248 molecule,CD248,CD164L1,CD164 sialomucin-like 1
蛋白构建
Gln18-Leu687
蛋白纯度
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
分子量72.7 kDa (predicted). Due to glycosylation, the protein migrates to 115-140 kDa based on Tris-Bis PAGE result.
内毒素< 1 EU/μg by the LAL method.
缓冲液Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization.
复溶方法
Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
存储
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式In general, Lyophilized powders are shipping with blue ice.
研究背景
Colorectal cancer (CRC) is one of the most common cancers worldwide usually diagnosed in the advanced stage. The serum concentration of tumor endothelial marker 1 (TEM1) was measured and correlated with clinicopathological features to evaluate whether TEM1 might serve as a biomarker for early CRC diagnosis, progression, and prognosis. TEM1 can act as a potential diagnostic, progression, and prognostic serum biomarker for patients with CRC; TEM1 might be a good supplement for commonly used markers CEA and Ca 19-9.

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