S100A12 Protein, Human, Recombinant (His)

S100A12 Protein, Human, Recombinant (His)

产品编号 TMPY-07055

S100A12 Protein, Human, Recombinant (His) is expressed in E. coli. The accession number is P80511.

规格	价格	库存	数量
100 μg	¥ 3,820	期货

大包装 & 定制

加入购物车

TargetMol 的所有产品仅用作科学研究或药证申报，不能被用于人体，我们不向个人提供产品和服务。请您遵守承诺用途，不得违反法律法规规定用于任何其他用途。

常见问题解答

问如果低于说明书给出的最高溶解度情况下，产品仍未溶解怎么办？

答建议您使用一些辅助溶解方法，如水浴加热至 45℃ 或用超声震荡，加速粉末溶解。

问抑制剂加 DMSO 配成母液之后，能否放在常温的环境保存1周？

答不建议这么做，母液配置好分装保存，建议存于 -20℃ 或 -80℃，尽量避免反复冻融。如果您订购的规格很大，可以考虑分装粉末保存，固体粉末是比较稳定的，-20℃ 可以保存三年。

问溶解度写的“mg/mL”和“mM”是什么意思？

答mg/mL 与 mM，是两种不同单位的表示方式，所指代的最大溶解度是一样的，可以通过官网网页下方的摩尔浓度计算器进行换算

问是否能直接用缓冲液对抑制剂 DMSO 母液做梯度稀释？

答大部分情况下，都是可以溶解的。但有时，有机试剂直接加入水相介质时会析出。建议将抑制剂先以DMSO做梯度稀释，再将经过稀释的抑制剂加入缓冲液或细胞培养基。有些抑制剂甚至只有在其工作浓度下才能溶于水相。比如细胞实验中希望终浓度为 1 μM 的话，可以把 10 mM 的 DMSO 母液用 DMSO 稀释到 1 mM，再吸 2 μL 加入到 2 mL 的生理盐水/PBS/细胞培液，终浓度即为 1 μM。为避免药物析出，稀释前，可将母液和培养基 37℃ 预热，避免温度低造成严重析出。若稀释过程中出现化合物析出的情况，建议您采用超声加热的方法使其复溶。

问超声一般需要多久？

答超声时长和溶解的量和浓度都有关，一般建议半个小时左右，如果浓度接近最大溶解度，或者需溶解的粉末较多，建议延长超声时间，并辅以加热。

产品信息

生物活性	Activity testing is in progress. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first.
产品描述	S100A12 Protein, Human, Recombinant (His) is expressed in E. coli. The accession number is P80511.
种属	Human
表达系统	E. coli
标签	C-His
蛋白编号	P80511
别名	p6,MRP-6,MRP6,ENRAGE,CGRP,CAGC,CAAF1
蛋白构建	A DNA sequence encoding the Human S100A12 (NP_005612.1) (Met1-Glu92) was expressed with a polyhistidine tag at the C-terminus.
蛋白纯度	≥ 95% as determined by SDS-PAGE. ≥ 95% as determined by SEC-HPLC.
分子量	11.4 kDa (predicted)
内毒素	< 1.0 EU per μg protein as determined by the LAL method.
缓冲液	Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
复溶方法	Please refer to the lot-specific COA.
存储	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式	In general, Lyophilized powders are shipping with blue ice.
研究背景	S100 protein is a family of low molecular weight protein found in vertebrates characterized by two EF-hand calcium-binding motifs. There are at least 21 different S100 proteins, and the name is derived from the fact that the protein is 100% soluble in ammonium sulfate at neutral pH. Most S100 proteins are disulfide-linked homodimer, and is normally present in cells derived from the neural crest, chondrocytes, macrophages, dendritic cells, etc. S100 proteins have been implicated in a variety of intracellular and extracellular functions. They are involved in regulation of protein phosphorylation, transcription factors, the dynamics of cytoskeleton constituents, enzyme activities, cell growth and differentiation, and the inflammatory response. Protein S100-A12, also known as S100 calcium-binding protein A12, Calcium-binding protein in amniotic fluid 1, Calgranulin-C, and S100A12, is a member of the S-101 family. Like the majority of S100 proteins, S100A12 is a dimer, with the interface between the two subunits being composed mostly of hydrophobic residues. The fold of S100A12 is similar to the other known crystal and solution structures of S100 proteins, except for the linker region between the two EF-hand motifs. S100A12 plays an important role in the inflammatory response.

S100A12 Protein, Human, Recombinant (His)

S100A12 Protein, Human, Recombinant (His)

产品信息

参考文献

SCI 文献

计算器

技术支持