RBP4 重组蛋白, Canine (His)

RBP4 Protein, Canine, Recombinant (His)

产品编号 TMPY-02861 RBP4 Protein, Canine, Recombinant (His)

RBP4 Protein, Canine, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 22.4 kDa and the accession number is F1PQI4.

规格	价格	库存	数量
100 μg	¥ 3,820	5日内发货

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常见问题解答

问是否能直接用缓冲液对抑制剂 DMSO 母液做梯度稀释？

答大部分情况下，都是可以溶解的。但有时，有机试剂直接加入水相介质时会析出。建议将抑制剂先以DMSO做梯度稀释，再将经过稀释的抑制剂加入缓冲液或细胞培养基。有些抑制剂甚至只有在其工作浓度下才能溶于水相。比如细胞实验中希望终浓度为 1 μM 的话，可以把 10 mM 的 DMSO 母液用 DMSO 稀释到 1 mM，再吸 2 μL 加入到 2 mL 的生理盐水/PBS/细胞培液，终浓度即为 1 μM。为避免药物析出，稀释前，可将母液和培养基 37℃ 预热，避免温度低造成严重析出。若稀释过程中出现化合物析出的情况，建议您采用超声加热的方法使其复溶。

问加药后检测结果没有作用，是不是产品质量有问题？

答产品质量没有问题，都是经过两次严格质检的，HNMR确定产品结构，HPLC确定化合物纯度。同一个抑制剂由于实验材料（细胞、动物）的不同，IC50 和实验效果也是不一样的，不能照搬文献报道的方法，应该根据自己的实验设立浓度梯度，得到一个最适合的浓度。

问动物不能耐受 DMSO，给药时 DMSO量应该如何控制？

答对于普通的老鼠，DMSO 的浓度应控制在 10% 以下，对于裸鼠、转基因小鼠、耐受性弱的老鼠等，DMSO 浓度需控制在2%以下。对于首次操作的抑制剂，建议先做溶剂阴性对照，确认溶解对动物无非特异性影响。

问超声一般需要多久？

答超声时长和溶解的量和浓度都有关，一般建议半个小时左右，如果浓度接近最大溶解度，或者需溶解的粉末较多，建议延长超声时间，并辅以加热。

问母液配置的计算方法？

答（1） c=n/v=m/M/v 浓度=物质的量/体积=质量/相对分子质量/体积（2）官网产品详情页下方有溶液配制表格，可以参考计算好的加入溶剂体积

产品信息

生物活性	Measured by its ability to bind alltrans retinoic acid. The binding of retinoic acid results in the quenching of Trp fluorescence in RBP4. The 50% binding concentration (BC50) is >1 µM
产品描述	RBP4 Protein, Canine, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 22.4 kDa and the accession number is F1PQI4.
种属	Canine
表达系统	HEK293 Cells
标签	C-His
蛋白编号	F1PQI4
别名	retinol binding protein 4, plasma
蛋白构建	A DNA sequence encoding the canine RBP4 (F1PQI4) (Met1-Leu201) was expressed with a C-terminal polyhistidine tag. Predicted N terminal: Glu 19
蛋白纯度	> 94 % as determined by SDS-PAGE
分子量	22.4 kDa (predicted); 23 kDa (reducing condition, due to glycosylation)
内毒素	< 1.0 EU/μg of the protein as determined by the LAL method.
缓冲液	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
复溶方法	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
存储	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式	In general, Lyophilized powders are shipping with blue ice.
研究背景	Retinol-binding protein 4 (RBP4) is the specific carrier for retinol (also known as vitamin A), and is responsible for the conversion of unstable and insoluble retinol in aqueous solution into stable and soluble complex in plasma through their tight interaction. As a member of the lipocalin superfamily, RBP4 containing a β-barrel structure with a well-defined cavity is secreted from the liver, and in turn delivers retinol from the liver stores to the peripheral tissues. In plasma, the RBP4-retinol complex interacts with transthyretin (TTR), and this binding is crucial for preventing RBP4 excretion through the kidney glomeruli. RBP4 expressed from an ectopic source efficiently delivers retinol to the eyes, and its deficiency affects night vision largely. Recently, RBP4 as an adipokine, is found to be expressed in adipose tissue and correlated with obesity, insulin resistance (IR) and type 2 diabetes (T2DM).

RBP4 Protein, Canine, Recombinant (His)

RBP4 Protein, Canine, Recombinant (His)

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