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Pal Protein, Legionella pneumophila, Recombinant (His)

产品编号 TMPH-02400

Part of the Tol-Pal system, which plays a role in outer membrane invagination during cell division and is important for maintaining outer membrane integrity. Very strongly associated with the peptidoglycan.

Pal Protein, Legionella pneumophila, Recombinant (His)

Pal Protein, Legionella pneumophila, Recombinant (His)

产品编号 TMPH-02400
Part of the Tol-Pal system, which plays a role in outer membrane invagination during cell division and is important for maintaining outer membrane integrity. Very strongly associated with the peptidoglycan.
规格价格库存数量
20 μg
¥ 2,570
20日内发货
100 μg
¥ 5,370
20日内发货
大包装 & 定制
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实验操作小课堂
常见问题解答
超声一般需要多久?
超声时长和溶解的量和浓度都有关,一般建议半个小时左右,如果浓度接近最大溶解度,或者需溶解的粉末较多,建议延长超声时间,并辅以加热。
抑制剂能否用于动物实验?
可以的,我们的抑制剂都可以用于动物/体内实验。但有些化合物还没有用于动物实验的文献,这种情况下,建议您先做预实验,确保研究的可行性。
如果低于说明书给出的最高溶解度情况下,产品仍未溶解怎么办?
建议您使用一些辅助溶解方法,如水浴加热至 45℃ 或用超声震荡,加速粉末溶解。
抑制剂是否可以高温灭菌?
大部分产品均通过化学方法合成,反应条件一般在 50-80℃ 之间,因此不建议使用高温灭菌法进行灭菌,建议用 0.22 μm 滤膜过滤灭菌。
加药后检测结果没有作用,是不是产品质量有问题?
产品质量没有问题,都是经过两次严格质检的,HNMR确定产品结构,HPLC确定化合物纯度。同一个抑制剂由于实验材料(细胞、动物)的不同,IC50 和实验效果也是不一样的,不能照搬文献报道的方法,应该根据自己的实验设立浓度梯度,得到一个最适合的浓度。
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产品信息

生物活性
Activity has not been tested. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first.
产品描述
Part of the Tol-Pal system, which plays a role in outer membrane invagination during cell division and is important for maintaining outer membrane integrity. Very strongly associated with the peptidoglycan.
种属
Legionella pneumophila
表达系统
P. pastoris (Yeast)
标签C-6xHis
蛋白编号P26493
别名
pplA,PPL,Peptidoglycan-associated lipoprotein,PAL,pal,19 kDa surface antigen
氨基酸序列
CSKTPGSADGGAAVGDGDATAQGLGQMTHFAGQEPGESYTTQAPHNQLYLFAYDDSTLASKYLPSVNAQAEYLKTHPGARVMIAGHTDERGSREYNVALGERRADTVAEILRMAGVSRQQIRVVSYGKERPANYGHDEASHAQNRRVEFIYEATR
蛋白构建
22-176 aa
蛋白纯度
> 85% as determined by SDS-PAGE.
分子量18.3 kDa (predicted)
内毒素< 1.0 EU/μg of the protein as determined by the LAL method.
缓冲液If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
复溶方法
Reconstitute the lyophilized protein in sterile deionized water. The product concentration should not be less than 100 μg/mL. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
存储
Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式In general, Lyophilized powders are shipping with blue ice. Solutions are shipping with dry ice.
研究背景
Part of the Tol-Pal system, which plays a role in outer membrane invagination during cell division and is important for maintaining outer membrane integrity. Very strongly associated with the peptidoglycan.

参考文献

SCI 文献

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