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PABPC1 Protein, Human, Recombinant (His & SUMO)

产品编号 TMPH-01885

PABPC1 Protein, Human, Recombinant (His & SUMO) is expressed in E. coli expression system with N-6xHis-SUMO tag. The predicted molecular weight is 57.8 kDa and the accession number is P11940.

PABPC1 Protein, Human, Recombinant (His & SUMO)

PABPC1 Protein, Human, Recombinant (His & SUMO)

产品编号 TMPH-01885
PABPC1 Protein, Human, Recombinant (His & SUMO) is expressed in E. coli expression system with N-6xHis-SUMO tag. The predicted molecular weight is 57.8 kDa and the accession number is P11940.
规格价格库存数量
20 μg
¥ 1,320
20日内发货
100 μg
¥ 2,680
20日内发货
1 mg
¥ 11,700
20日内发货
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实验操作小课堂
常见问题解答
抑制剂能否用于动物实验?
可以的,我们的抑制剂都可以用于动物/体内实验。但有些化合物还没有用于动物实验的文献,这种情况下,建议您先做预实验,确保研究的可行性。
是否能直接用缓冲液对抑制剂 DMSO 母液做梯度稀释?
大部分情况下,都是可以溶解的。但有时,有机试剂直接加入水相介质时会析出。建议将抑制剂先以DMSO做梯度稀释,再将经过稀释的抑制剂加入缓冲液或细胞培养基。有些抑制剂甚至只有在其工作浓度下才能溶于水相。 比如细胞实验中希望终浓度为 1 μM 的话,可以把 10 mM 的 DMSO 母液用 DMSO 稀释到 1 mM,再吸 2 μL 加入到 2 mL 的生理盐水/PBS/细胞培液,终浓度即为 1 μM。 为避免药物析出,稀释前,可将母液和培养基 37℃ 预热,避免温度低造成严重析出。若稀释过程中出现化合物析出的情况,建议您采用超声加热的方法使其复溶。
抑制剂是否可以高温灭菌?
大部分产品均通过化学方法合成,反应条件一般在 50-80℃ 之间,因此不建议使用高温灭菌法进行灭菌,建议用 0.22 μm 滤膜过滤灭菌。
化合物如何用于动物实验?如何确立剂量、给药方式等?
同一个化合物对不同动物模型、不同疾病模型的用法不一样,建议根据实验目的,查询文献选择用法。 常见的给药方式包括腹腔注射、灌胃等。由于首过效应,一般可以灌胃的也可以腹腔注射,可以腹腔注射的不一定适合灌胃。不同种属的给药剂量可以根据官网的表格换算。但仅供参考,实际应用中可能未必合适或者准确,尽量以具体某种动物给药剂量的参考文献为主。 我们的化合物都可以进行细胞动物实验,但有些化合物还没有用于动物实验的文献,这种情况下,您可以尝试进行动物实验,但我们无法提供使用方式,也无法保证药效。
收到粉状试剂粘壁严重,如何处理?
建议您用离心机 3000 rpm下离心一会儿,将粉末收集到管底。
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产品信息

生物活性
Activity has not been tested. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first.
产品描述
PABPC1 Protein, Human, Recombinant (His & SUMO) is expressed in E. coli expression system with N-6xHis-SUMO tag. The predicted molecular weight is 57.8 kDa and the accession number is P11940.
种属
Human
表达系统
E. coli
标签N-6xHis-SUMO
蛋白编号P11940
别名
Polyadenylate-binding protein 1,Poly(A)-binding protein 1,PABPC2,PABPC1,PABP-1,PABP1,PABP,PAB1
氨基酸序列
MNPSAPSYPMASLYVGDLHPDVTEAMLYEKFSPAGPILSIRVCRDMITRRSLGYAYVNFQQPADAERALDTMNFDVIKGKPVRIMWSQRDPSLRKSGVGNIFIKNLDKSIDNKALYDTFSAFGNILSCKVVCDENGSKGYGFVHFETQEAAERAIEKMNGMLLNDRKVFVGRFKSRKEREAELGARAKEFTNVYIKNFGEDMDDERLKDLFGKFGPALSVKVMTDESGKSKGFGFVSFERHEDAQKAVDEMNGKELNGKQIYVGRAQKKVERQTELKRKFEQMKQDRITRYQGVNLYVKNLDDGIDDERLRKEFSPFGTITSAKVMMEGGRSKGFGFVCFSSPEEATKAVTEMNGRIVATKPLYVALAQR
蛋白构建
1-370 aa
蛋白纯度
> 90% as determined by SDS-PAGE.
分子量57.8 kDa (predicted)
内毒素< 1.0 EU/μg of the protein as determined by the LAL method.
缓冲液Tris-based buffer, 50% glycerol
复溶方法
A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
存储
Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式In general, Lyophilized powders are shipping with blue ice. Solutions are shipping with dry ice.
研究背景
Binds the poly(A) tail of mRNA, including that of its own transcript, and regulates processes of mRNA metabolism such as pre-mRNA splicing and mRNA stability. Its function in translational initiation regulation can either be enhanced by PAIP1 or repressed by PAIP2. Can probably bind to cytoplasmic RNA sequences other than poly(A) in vivo. Involved in translationally coupled mRNA turnover. Implicated with other RNA-binding proteins in the cytoplasmic deadenylation/translational and decay interplay of the FOS mRNA mediated by the major coding-region determinant of instability (mCRD) domain. Involved in regulation of nonsense-mediated decay (NMD) of mRNAs containing premature stop codons; for the recognition of premature termination codons (PTC) and initiation of NMD a competitive interaction between UPF1 and PABPC1 with the ribosome-bound release factors is proposed. By binding to long poly(A) tails, may protect them from uridylation by ZCCHC6/ZCCHC11 and hence contribute to mRNA stability.; (Microbial infection) Positively regulates the replication of dengue virus (DENV).

参考文献

SCI 文献

计算器

  • 复溶 计算器
  • 重组蛋白稀释 计算器
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