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Oncostatin M/OSM Protein, Human, Recombinant

产品编号 TMPY-00283
Oncostatin M/OSM Protein, Human, Recombinant is expressed in HEK293 mammalian cells. The predicted molecular weight is 22.16 kDa and the accession number is P13725.
规格价格库存数量
20 μg
¥ 1,950
现货
100 μg
¥ 4,920
5日内发货
1 mg
¥ 28,800
5日内发货
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产品信息

生物活性
Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is typically 0.3-1.2ng/mL.
产品描述
Oncostatin M/OSM Protein, Human, Recombinant is expressed in HEK293 mammalian cells. The predicted molecular weight is 22.16 kDa and the accession number is P13725.
种属
Human
表达系统
HEK293 Cells
标签Tag Free
蛋白编号P13725
别名
oncostatin M
蛋白构建
A DNA sequence encoding the human OSM (NP_065391.1) (Met1-Arg221) was expressed. Predicted N terminal: Ala 26
蛋白纯度
≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.
分子量22.16 kDa (predicted); 32 kDa (reducing condition, due to glycosylation)
内毒素< 10 EU/mg of the protein.
缓冲液Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
复溶方法
A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
存储
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式In general, Lyophilized powders are shipping with blue ice.
研究背景
Oncostatin M (OSM) is a glycoprotein belonging to the interleukin-6 family of cytokines that has functions mainly in cell growth. Oncostatin M (OSM) is considered as a pleiotropic cytokine that signals through cell surface receptors typeⅠand typeⅡ both of which share the similarity of containing protein gp130 and takes part in many bio metabolism processes including liver development, hematopoiesis, inflammation, bone formation, and destruction and possibly CNS development. Oncostatin M (OSM) was previously identified by its ability to inhibit the growth of cells from melanoma and other solid tumors. It also has been reported that OSM, like LIF, IL-6, and G-CSF, can inhibit the proliferation of murine M1 myeloid leukemic cells and can induce their differentiation into macrophage-like cells. The human form of OSM is insensitive between pH2 and 11 and resistant to heating for one hour at 56 degrees but is not stable at 90 degrees. The human OSM is produced as a precursor containing 252 amino acids, whose first 25 amino acids function as a secretory signal peptide and which on removal yields the soluble 227 amino acid pro-OSM. Removal of the C-terminal most 31 amino acids produces the fully active 196 residue form.

参考文献

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