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MSLN/Mesothelin Protein, Human, Recombinant (His), AF488-Labeled

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货号 TMPY-07108

别名 SMRP, MPF, CAK1

MSLN/Mesothelin Protein, Human, Recombinant (His), AF488-Labeled is expressed in HEK293 Cells. The accession number is Q13421.

MSLN/Mesothelin Protein, Human, Recombinant (His), AF488-Labeled
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MSLN/Mesothelin Protein, Human, Recombinant (His), AF488-Labeled

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SPR兼容缓冲液
货号 TMPY-07108 别名 SMRP, MPF, CAK1

MSLN/Mesothelin Protein, Human, Recombinant (His), AF488-Labeled is expressed in HEK293 Cells. The accession number is Q13421.

规格价格库存数量
5 μg
¥ 1,330
6-8日内发货
10 μg
¥ 2,230
6-8日内发货
25 μg
¥ 4,460
5日内发货
50 μg
¥ 7,850
5日内发货
100 μg
¥ 16,500
5日内发货
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产品介绍

生物活性
产品描述
MSLN/Mesothelin Protein, Human, Recombinant (His), AF488-Labeled is expressed in HEK293 Cells. The accession number is Q13421.
生物活性
1. Flow cytometric analysis of anti-Mesothelin CAR expression. 293 cells were transiently transduced with anti-Mesothelin CAR. Flow cytometric analysis was performed with a negative control protein and MSLN/Mesothelin Protein, Human, Recombinant (His), AF488-Labeled (Cat#TMPY-07108), respectively. Non-transduced 293 cells were used as a control (left).
2. Binding activity of AF 488-conjugated Mesothelin protein to PBMC cells. PBMC cells were stained with anti-CD3 antibody and MSLN/Mesothelin Protein, Human, Recombinant (His), AF488-Labeled (Cat#TMPY-07108) and detected by flow cytometry. PBMC cells stained with anti-CD3 antibody were used as a control (left).
研究背景
The megakaryocyte potentiating factor belongs to the mesothelin family. This family is comprised of several mammalian pre-pro-megakaryocyte potentiating factor precursor (MPF) or mesothelin proteins. Mesothelin is a glycosylphosphatidylinositol-linked glycoprotein highly expressed in mesothelial cells, mesotheliomas, and ovarian cancer, but the biological function of the protein is not known. Megakaryocyte potentiating factor is highly expressed in mesotheliomas, ovarian cancers, and some squamous cell carcinomas (at protein level). It interacts with MUC16 and potentiates megakaryocyte colony formation in vitro. Megakaryocyte potentiating factor is secreted by several mesothelioma cell lines and is frequently elevated in the blood of patients with mesothelioma. Measurement of this protein may be useful in following the response of mesothelioma to treatment.Cancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy
种属
Human
表达系统
HEK293 Cells
标签C-His
蛋白编号Q13421
蛋白构建A DNA sequence encoding the human MSLN (AAH09272.1) (Glu296-Gly580) was expressed with a C-terminal polyhistidine tag. The protein is site-specifically conjugated with AF 488 (Excitation Max.= 495 nm, Emission Max.= 519 nm).
缓冲液This product is Lyophilized from sterile PBS, 5% Trehalose, 5% Mannitol, pH 7.4. Please contact us for any concerns or special requirements. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
复溶方法Please refer to the lot-specific COA.
别名SMRP, MPF, CAK1
内毒素< 1.0 EU per μg protein as determined by the LAL method.
化学信息
分子量38 kDa (predicted)
储存&溶解度
运输方式In general, Lyophilized powders are shipping with blue ice.
存储Twelve months from date of receipt at -20℃ to -70℃ in lyophilized form and 3 months at -70℃ under sterile conditions after reconstitution. Protect from prolonged exposure to light and avoid repeated freeze-thaw cycles.

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