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KCNE2 Protein, Human, Recombinant (His)

产品编号 TMPH-01891

KCNE2 Protein, Human, Recombinant (His) is expressed in E. coli expression system with N-10xHis tag. The predicted molecular weight is 17.3 kDa and the accession number is Q9Y6J6.

KCNE2 Protein, Human, Recombinant (His)

KCNE2 Protein, Human, Recombinant (His)

产品编号 TMPH-01891
KCNE2 Protein, Human, Recombinant (His) is expressed in E. coli expression system with N-10xHis tag. The predicted molecular weight is 17.3 kDa and the accession number is Q9Y6J6.
规格价格库存数量
20 μg
¥ 9,600
20日内发货
100 μg
¥ 17,600
20日内发货
大包装 & 定制
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实验操作小课堂
常见问题解答
如何设置母液浓度?
母液浓度需低于官网给出的溶解度,在这个范围内,根据工作液浓度设置母液浓度,细胞实验中,建议母液浓度设定在工作液浓度的 1000 倍以上。
化合物如何用于动物实验?如何确立剂量、给药方式等?
同一个化合物对不同动物模型、不同疾病模型的用法不一样,建议根据实验目的,查询文献选择用法。 常见的给药方式包括腹腔注射、灌胃等。由于首过效应,一般可以灌胃的也可以腹腔注射,可以腹腔注射的不一定适合灌胃。不同种属的给药剂量可以根据官网的表格换算。但仅供参考,实际应用中可能未必合适或者准确,尽量以具体某种动物给药剂量的参考文献为主。 我们的化合物都可以进行细胞动物实验,但有些化合物还没有用于动物实验的文献,这种情况下,您可以尝试进行动物实验,但我们无法提供使用方式,也无法保证药效。
收到粉状试剂粘壁严重,如何处理?
建议您用离心机 3000 rpm下离心一会儿,将粉末收集到管底。
期货产品没有纯度啊?这个纯度在哪里看
期货产品的话,是没有纯度信息,也没有质检信息的。有了现货之后我们会进行质检,测出试剂的纯度和结构等相关信息,并且展示在官网的活性描述上方。
超声久了会不会对化合物的结构有影响?
建议以较低频率对化合物进行超声,不会对化合物的结构造成影响。
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产品信息

生物活性
Activity has not been tested. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first.
产品描述
KCNE2 Protein, Human, Recombinant (His) is expressed in E. coli expression system with N-10xHis tag. The predicted molecular weight is 17.3 kDa and the accession number is Q9Y6J6.
种属
Human
表达系统
E. coli
标签N-10xHis
蛋白编号Q9Y6J6
别名
Potassium voltage-gated channel subfamily E member 2,Potassium channel subunit beta MiRP1,MinK-related peptide 1 (MiRP1),Minimum potassium ion channel-related peptide 1,KCNE2
氨基酸序列
MSTLSNFTQTLEDVFRRIFITYMDNWRQNTTAEQEALQAKVDAENFYYVILYLMVMIGMFSFIIVAILVSTVKSKRREHSNDPYHQYIVEDWQEKYKSQILNLEESKATIHENIGAAGFKMSP
蛋白构建
1-123 aa
蛋白纯度
> 90% as determined by SDS-PAGE.
分子量17.3 kDa (predicted)
内毒素< 1.0 EU/μg of the protein as determined by the LAL method.
缓冲液If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
复溶方法
Reconstitute the lyophilized protein in sterile deionized water. The product concentration should not be less than 100 μg/mL. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
存储
Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式In general, Lyophilized powders are shipping with blue ice. Solutions are shipping with dry ice.
研究背景
Ancillary protein that assembles as a beta subunit with a voltage-gated potassium channel complex of pore-forming alpha subunits. Modulates the gating kinetics and enhances stability of the channel complex. Assembled with KCNB1 modulates the gating characteristics of the delayed rectifier voltage-dependent potassium channel KCNB1. Associated with KCNH2/HERG is proposed to form the rapidly activating component of the delayed rectifying potassium current in heart (IKr). May associate with KCNQ2 and/or KCNQ3 and modulate the native M-type current. May associate with HCN1 and HCN2 and increase potassium current. Interacts with KCNQ1; forms a heterooligomer complex leading to currents with an apparently instantaneous activation, a rapid deactivation process and a linear current-voltage relationship and decreases the amplitude of the outward current.

参考文献

SCI 文献

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