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IL-1R2 Protein, Canine, Recombinant (hFc)

产品编号 TMPY-03904

IL-1R2 Protein, Canine, Recombinant (hFc) is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 64.8 kDa and the accession number is E2RKW1.

IL-1R2 Protein, Canine, Recombinant (hFc)

IL-1R2 Protein, Canine, Recombinant (hFc)

产品编号 TMPY-03904
IL-1R2 Protein, Canine, Recombinant (hFc) is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 64.8 kDa and the accession number is E2RKW1.
规格价格库存数量
50 μg
¥ 2,530
5日内发货
大包装 & 定制
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实验操作小课堂
常见问题解答
细胞实验中需要加多少抑制剂?
通常建议您参考同模型实验发表的文献报道;除参考文献报道以外,还需要通过预实验做“剂量-效应曲线”确定最佳作用浓度(浓度梯度);通过“时间-效应曲线”确定最佳孵育时间(处理时间梯度)。另外,抑制剂的使用量受多种因素影响,包括抑制对象的可接触性、细胞通透性、孵育时间、细胞种类等等。我们建议通过检索文献确定使用抑制剂的起始浓度。 如果有报道的 Ki 值或 IC50 值,可以采用其 5-10 倍的量开始尝试以达到抑制酶活性的最佳效果。 如果抑制剂的 Ki 值或 IC50 值未知,则需要在更广泛的范围尝试抑制剂的使用浓度,并采用 Michaelis-Menten 动力学计算 Ki 值。 一般设立溶解抑制剂时所采用的溶剂作为对照,以排除溶剂的非特异性影响。
超声一般需要多久?
超声时长和溶解的量和浓度都有关,一般建议半个小时左右,如果浓度接近最大溶解度,或者需溶解的粉末较多,建议延长超声时间,并辅以加热。
产品是否需要避光?
一般来说,如果有需要避光储存的分子,我们会选择棕色玻璃瓶发货的。
动物实验常用溶解方法?
首先,您需要确认给药剂量、给药方式。对于具体的产品,优先找引用我们产品的文献里的使用方法,再找其他文献里的配方。 如果没有相关的文献,且该化合物的 DMSO 溶解度比较好,我们推荐通用配方: 10% DMSO+40% PEG300+5% Tween-80+45% Saline/PBS/ddH2O。溶剂依次加入,尽量溶解后再加入下一个溶剂。正常鼠建议 DMSO 浓度在 10% 以下,裸鼠体弱鼠等配方的 DMSO 浓度建议在 2% 以下,根据溶液是否澄清,助溶剂 PEG300、Tween-80 的比例可以适当调整。如果有其他助溶剂也可以使用。 以上配方仅供参考,体内配方并不是绝对的,需要根据不同情况进行调整。建议先取少量化合物测试配方,再进行大量配制。配完也可以再使用超声、加热等方式助溶,看看能不能澄清一点。 腹腔注射对粉末的溶解度要求比较高,建议购买盐形式化合物。如果给药剂量比较大,也有报道使用混悬液进行腹腔给药的。 对于灌胃给药,且剂量比较大的情况下,建议用 0.5% CMC-Na 配置成均匀混悬液进行给药。
抑制剂是否可以高温灭菌?
大部分产品均通过化学方法合成,反应条件一般在 50-80℃ 之间,因此不建议使用高温灭菌法进行灭菌,建议用 0.22 μm 滤膜过滤灭菌。
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产品信息

生物活性
Measured by its binding ability in a functional ELISA. Immobilized Canine IL1R2-Fc3 at 10 μg/ml (100 μl/well) can bind biotinylated human IL1B-His , The EC50 of biotinylated human IL1B-His is 0.08-0.2 μg/ml.
产品描述
IL-1R2 Protein, Canine, Recombinant (hFc) is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 64.8 kDa and the accession number is E2RKW1.
种属
Canine
表达系统
HEK293 Cells
标签C-hFc
蛋白编号E2RKW1
别名
interleukin 1 receptor, type II
蛋白构建
A DNA sequence encoding the canine IL1R2 (E2RKW1) (Met1-Glu343) was expressed with the Fc region of human IgG1 at the C-terminus. Predicted N terminal: Phe 14
蛋白纯度
> 90 % as determined by SDS-PAGE
分子量64.8 kDa (predicted); 65-70 kDa (reducing conditions)
内毒素< 1.0 EU/μg of the protein as determined by the LAL method.
缓冲液Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
复溶方法
A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
存储
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式In general, Lyophilized powders are shipping with blue ice.
研究背景
Interleukin 1 receptor, type II (IL1R2) also known as CD121b (Cluster of Differentiation 121b) is a cytokine receptor that belongs to the interleukin-1 receptor family. This protein binds interleukin alpha (IL1A), interleukin beta (IL1B), and interleukin 1 receptor, type I (IL1R1/IL1RA), and acts as a decoy receptor that inhibits the activity of its ligands. The pleiotropic cytokine IL1 is produced to regulate the development and maintenance of the inflammatory responses and binds to specific plasma membrane receptors on cells. Two distinct types of IL1 receptors that can bind IL1 specifically have been identified, designated as IL1RI (IL1RA) and IL1RII (IL1RB). IL1R1 contributes to IL-1 signaling, whereas the IL-1R2/CD121b has no signaling property and acts as a decoy for IL-1. IL-1R2/CD121b structurally consisting of a ligand-binding portion comprised of three Ig-like domains, a single transmembrane region, and a short cytoplasmic domain is expressed in a variety of cell types including B lymphocytes, neutrophils, monocytes, large granular leukocytes, and endothelial cells. Interleukin 4 (IL4) is reported to antagonize the activity of interleukin 1 by inducing the expression and release of this cytokine.

SCI 文献

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