HER2/ERBB2 Protein, Human, Recombinant (His & Avi), Biotinylated

HER2/ERBB2 Protein, Human, Recombinant (His & Avi), Biotinylated is expressed in HEK293 mammalian cells with His and Avi tag. The predicted molecular weight is 72.6 kDa and the accession number is P04626-1.

规格	价格/CNY		货期	数量
20 μg	￥ 2,940	5日内发货
100 μg	￥ 6,800	5日内发货

规格

价格/CNY

货期

数量

20 μg

￥ 2,940

5日内发货

100 μg

￥ 6,800

5日内发货

生物活性

1. Immobilized Anti-Erbb2 Antibody (Trastuzumab), Human IgG1 at 2μg/mL (100μL/well) can bind Recombinant Human HER2 / ErbB2 Protein (His & AVI Tag), Biotinylated , the EC50 is 1-3 ng/mL. 2. Measured by its ability to block anti-ErbB2 mediated inhibition of BT474 human breast ductal carcinoma cell proliferation. The ED50 for this effect is 0.1-0.5 μg/mL in the presence of 0.6 µg/mL Anti-ErbB2/Her2 Monoclonal Antibody (Routinely tested).

产品描述

种属	Human
表达系统	HEK293 Cells
标签	His, Avi
蛋白编号	P04626-1
别名	ERVWE1, HER-2/neu, NEU, HERV-7q, erb-b2 receptor tyrosine kinase 2, MLN19, HERVWENV, NGL, HERV7Q, EGFR2, ENV, TKR1, HER-2, CD340, HERVW, ENVW, HERV-W-ENV, MLN 19, HER2
蛋白构建	The Human ERBB2 (NP_004439.2) (Met1-Thr652) was expressed with a c-terminal polyhistidine tagged AVI tag at the C-terminus. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed.
蛋白纯度	≥ 90 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC.
分子量	72.6 kDa (predicted)

内毒素	< 1.0 EU/μg of the protein as determined by the LAL method.
缓冲液	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
复溶方法	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
存储	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式	In general, Lyophilized powders are shipping with blue ice.
研究背景	Human epidermal growth factor receptor 2 (HER2), also known as ErbB2, NEU, and CD340, is a type I membrane glycoprotein and belongs to the epidermal growth factor (EGF) receptor family. HER2 protein cannot bind growth factors due to the lacking of ligand binding domain of its own and autoinhibited constitutively. However, HER2 forms a heterodimer with other ligand-bound EGF receptor family members, therefore stabilizes ligand binding and enhances kinase-mediated activation of downstream molecules. HER2 plays a key role in development, cell proliferation and differentiation. HER2 gene has been reported to associate with malignancy and a poor prognosis in numerous carcinomas, including breast, prostate, ovarian, lung cancers and so on.Cancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy