Name: HER2/ERBB2 Protein, Human, Recombinant (aa 1-652, His)
Brand: TargetMol
SKU: TMPY-01137
Price: 373 CNY
Availability: InStock
Rating: 5 (10 reviews)

规格

价格

库存

数量

5 μg

¥ 373

现货

10 μg

¥ 588

现货

20 μg

¥ 986

现货

50 μg

¥ 1,930

现货

100 μg

¥ 3,260

现货

200 μg

¥ 5,770

5日内发货

500 μg

¥ 12,200

5日内发货

生物活性

产品描述	HER2/ERBB2 Protein, Human, Recombinant (aa 1-652, His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 71 kDa and the accession number is P04626-1.
生物活性	1. Immobilized Her2/ERBB2 Protein, Human, Recombinant (ECD, His Tag) at 2 μg/ml (100 μl/well) can bind Anti-Erbb2 Antibody (Trastuzumab), the EC50 is 10-60 ng/mL. 2. Measured by its ability to block anti-ErbB2 mediated inhibition of BT474 human breast ductal carcinoma cell proliferation. The ED50 for this effect is 0.2-1 μg/mL in the presence of 0.6 µg/mL Anti-ErbB2/Her2 Monoclonal Antibody. 3. Using the Octet RED System, the affinity constant (Kd) of Her2 / ERBB2 Protein, Human, Recombinant (His Tag) bound to Pertuzumab was 0.1 nM. 4.Captured anti-Her2 IgG1 antibody on proA Chip can bind Her2 with an affinity constant of 3.270 nM as determined in a SPR assay (Routinely tested). 5.Captured Trastuzumab on proA Chip can bind Her2 with an affinity constant of 3.727 nM as determined in a SPR assay(Routinely tested).
研究背景	Human epidermal growth factor receptor 2 (HER2), also known as ErbB2, NEU, and CD340, is a type I membrane glycoprotein and belongs to the epidermal growth factor (EGF) receptor family. HER2 protein cannot bind growth factors due to the lacking of ligand binding domain of its own and autoinhibited constitutively. However, HER2 forms a heterodimer with other ligand-bound EGF receptor family members, therefore stabilizes ligand binding and enhances kinase-mediated activation of downstream molecules. HER2 plays a key role in development, cell proliferation and differentiation. HER2 gene has been reported to associate with malignancy and a poor prognosis in numerous carcinomas, including breast, prostate, ovarian, lung cancers and so on.Cancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy
种属	Human
表达系统	HEK293 Cells
标签	C-His
蛋白编号	P04626-1
蛋白构建	A DNA sequence encoding the extracellular domain (Met 1-Thr 652) of human ErbB2 (NP_004439.2) was fused with the polyhistidine tag at the C-terminus. Predicted N terminal: Thr 23
蛋白纯度	> 90 % as determined by SDS-PAGE. > 90 % as determined by SEC-HPLC.
蛋白性状	Lyophilized powder
缓冲液	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
复溶方法	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
别名	TKR1, NGL, NEU, MLN19, MLN 19, HERV-W-ENV, HERVWENV, HERVW, HERV-7q, HERV7Q, HER-2/neu, HER-2, HER2, ERVWE1, erb-b2 receptor tyrosine kinase 2, ENVW, ENV, EGFR2, CD340
内毒素	< 1.0 EU/μg of the protein as determined by the LAL method.