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HEPACAM2 Protein, Rat, Recombinant (hFc)

产品编号 TMPY-03586

HEPACAM2 Protein, Rat, Recombinant (hFc) is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 62.3 kDa and the accession number is B5DEN8.

HEPACAM2 Protein, Rat, Recombinant (hFc)

HEPACAM2 Protein, Rat, Recombinant (hFc)

产品编号 TMPY-03586
HEPACAM2 Protein, Rat, Recombinant (hFc) is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 62.3 kDa and the accession number is B5DEN8.
规格价格库存数量
100 μg
¥ 4,460
5日内发货
大包装 & 定制
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实验操作小课堂
常见问题解答
产品溶解需要超声吗?如果没有超声仪怎么办?
超声可以加快粉末的溶解速度,如果您的化合物溶解不了,建议您尝试超声。如果您没有超声仪,建议您选择较低母液浓度,或者较少粉末量,尝试溶解。
如何设置母液浓度?
母液浓度需低于官网给出的溶解度,在这个范围内,根据工作液浓度设置母液浓度,细胞实验中,建议母液浓度设定在工作液浓度的 1000 倍以上。
收到产品发现冰盒都化了,怎么办?
一般化合物粉末对温度不敏感,加冰盒是为了防止极端气温,如果冰盒融化不影响产品质量。
是否能直接用缓冲液对抑制剂 DMSO 母液做梯度稀释?
大部分情况下,都是可以溶解的。但有时,有机试剂直接加入水相介质时会析出。建议将抑制剂先以DMSO做梯度稀释,再将经过稀释的抑制剂加入缓冲液或细胞培养基。有些抑制剂甚至只有在其工作浓度下才能溶于水相。 比如细胞实验中希望终浓度为 1 μM 的话,可以把 10 mM 的 DMSO 母液用 DMSO 稀释到 1 mM,再吸 2 μL 加入到 2 mL 的生理盐水/PBS/细胞培液,终浓度即为 1 μM。 为避免药物析出,稀释前,可将母液和培养基 37℃ 预热,避免温度低造成严重析出。若稀释过程中出现化合物析出的情况,建议您采用超声加热的方法使其复溶。
抑制剂产品应如何储存?
粉末储存在 -20℃,可以存 3 年以上。 母液一般储存于 -80℃,可以存 1 年以上,建议多分装几管,避免反复冻融。 常用的存于 4℃,可放一周以上。
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产品信息

生物活性
Activity testing is in progress. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first.
产品描述
HEPACAM2 Protein, Rat, Recombinant (hFc) is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 62.3 kDa and the accession number is B5DEN8.
种属
Rat
表达系统
HEK293 Cells
标签C-hFc
蛋白编号B5DEN8
别名
HepaCAM2,HEPACAM family member 2
蛋白构建
A DNA sequence encoding the rat HepaCAM2 (B5DEN8) (Met1-Leu350) was expressed with the Fc region of human IgG1 at the C-terminus. Predicted N terminal: Leu 33
蛋白纯度
> 95 % as determined by SDS-PAGE
分子量62.3 kDa (predicted); 66-73 kDa (reducing conditions)
内毒素< 1.0 EU/μg of the protein as determined by the LAL method.
缓冲液Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
复溶方法
A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
存储
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式In general, Lyophilized powders are shipping with blue ice.
研究背景
HEPACAM2 (HEPACAM Family Member 2) is a Protein Coding gene. This gene encodes a protein related to the immunoglobulin superfamily that plays a role in mitosis. Knockdown of this gene results in prometaphase arrest, abnormal nuclear morphology, and apoptosis. HepaCAM2, together with HEPACAM1, are cell adhesion molecules. HEPACAM1 modulates cell adhesion and migration. It also inhibits cancer cell growth. HepaCAM2 contains 2 Ig-like C2-type (immunoglobulin-like) domains. The immunoglobulin domain is a type of protein domain that consists of a 2-layer sandwich of between 7 and 9 antiparallel β-strands arranged in two β-sheets with a Greek key topology. Members of the immunoglobulin superfamily are found in hundreds of proteins of different functions.

参考文献

SCI 文献

计算器

  • 复溶 计算器
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